Human Erythropoietin, EPO ELISA Kit

Sample

serum, plasma, other biological fluids

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of Human EPO in serum, plasma and other biological fluids.

Contents of Kit

1. Human EPO Microplate: polystyrene microplate coated with a monoclonal antibody against Human EPO;
2. Standard (freeze dried): 200 mLU/mL, 2 vials;
3. Biotin-antibody (100x): 2 vials;
4. HRP-avidin (100x): 2 vials;
5. Reagent Diluent: 25 mL, 2 vials;
6. TMB Substrate: 12 mL, 1 vial;
7. TMB Stop Solution: 1 vial;
8. Wash Buffer (20x): 30 mL, 1 vial;
9. Microtiter plate sealers: 3 sheets.

Storage

##Unopened Kit: ##Store at 2-8°C. Do not use past kit expiration date.
##Opened/Reconstituted Reagents: ## Standard or Sample Diluent; Biotin-antibody Diluent; HRP-avidin Diluent; TMB Substrate; Wash Buffer; TMB Stop Solution. The above mentioned reagents should be stored for up to 1 month at 2-8°C.
##Microplate Wells: ##Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2-8°C.

Sensitivity

##Detection Rage: ##1.56-100 mLU/mL.
##Detection Limit: ##The minimum detectable dose of Human EPO is typically less than 0.4 mlU/mL.