Biotinylated Human ACE2 Protein [His,Avi] (DAGC228)

Biotinylated Recombinant Human ACE2 Protein from HEK293 Cells [His,Avi]

Product Overview
Biotinylated Human ACE2, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Nature
Recombinant
Predicted N terminal
Gln 18
Tag/Conjugate
His,Avi
Molecular Weight
The protein has a calculated MW of 87.2 kDa. The protein migrates as 95-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Alternative Names
ACE2; ACHE; ACE-2
Procedure
None
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Format
Liquid
Size
25ug, 200ug
Buffer
Delivered as bulk protein in a 0.2 um filtered solution of 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.
Preservative
None
Storage
Store at -70°C or lower upon receipt.
Ship
Shipped with dry ice
Introduction
Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.
Keywords
ACE2; ACHE; ACE-2

Citations


Have you cited DAGC228 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


Kam, YW; Kien, F; et al. Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate Fc gamma RII- dependent entry into B cells in vitro. VACCINE 25:729-740(2007).
Lavillette, D; Barbouche, R; et al. Significant redox insensitivity of the functions of the SARS-CoV spike glycoprotein - Comparison with HIV envelope. JOURNAL OF BIOLOGICAL CHEMISTRY 281:9200-9204(2006).

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket