Anti-ACE2 monoclonal antibody (DCABH-120)

Rabbit Anti-ACE2 monoclonal antibody for WB, ICC, IHC, IP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TO1865
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:100-1:500; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of ACE2 on human kidney lysates using anti-ACE2 antibody at 1/1,000 dilution.
Fig2: ICC staining ACE2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ACE2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining ACE2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ACE2 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACE2 antibody. Counter stained with hematoxylin.

Target


Alternative Names
ACE 2; ACE related carboxypeptidase; ACE-related carboxypeptidase; ACE2; ACE2_HUMAN; ACEH
Entrez Gene ID

Citations


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References


Simmons, G; Bertram, S; et al. Different host cell proteases activate the SARS-coronavirus spike-protein for cell-cell and virus-cell fusion. VIROLOGY 413:265-274(2011).
Ho, TY; Wu, SL; et al. Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 interaction. ANTIVIRAL RESEARCH 74:92-101(2007).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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