Hepatitis A-Virus (HAV) Antigen ELISA Kit

Sample

Stool and cell culture

Detection Method

sELISA

Intended Use

The HAV Antigen ELISA Kit is an enzyme immunoassay for research use for the identification of Hepatitis A virus in stool and cell culture.

Contents of Kit

1. Microtiter plate: ready for use, coated with anti-HAV antigen-antibody. Wells are separately breakable. (8x12) wells
2. Conjugate Concentrate (flask A): 100-fold concentrate, contains the peroxidase conjugated mouse anti-HAV IgG. 1 x 150 μL
3. Positive Control (flask B): Hepatitis A-Virus antigen, inactivated, ready for use. 1 x 500 μL
4. Neutralising serum (flask C): anti-HAV-positive serum, 10× concentrated. 1 x 500 μL
5. Dilution buffer (flask D): ready for use, red colored. Dilution buffer for specimen und
conjugate, red coloured. 1 x 120 mL
6. Substrate (flask E): ready for use. 1 x 12 mL
7. Stop solution (flask F): ready for use, 0.2 M sulfuric acid. 1 x 12 mL
8. Wash buffer (flask G): 20-fold concentrated solution. 1 x 50 mL
9. Sealing Tape: for covering the microtiter plate. 2

Storage

All materials must be stored at 2 - 8°C in the dark. Unused microtiterplate stripes have to be stored airtight together with the desiccant bag at 2 - 8°C.
The shelf-life of the components after initial opening is guaranteed for four weeks.

Precision

Cut-off: 16 % CV intra-assay
Positive control: 3.5 % CV intra-assay

Sensitivity

91 % of the Elisa-positive samples were also HAV-PCR positive.

General Description

A positive identification of Hepatitis A Virus (HAV) in human stool samples indicates a fresh and contagious infection with HAV. The passing begins about two weeks before the icteric phase of the disease and reached a peak after about one week before icterus. With the beginning of the icteric phase, the HAV passing drops steeply but HAV antigen could be found in the stool of some, not all, humans two weeks after onset of icterus.
The detection of HAV in specimen other than stool is also possible with the CD HAV Antigen ELISA Kit, for example in lysates of HAV infected cells or in culture supernatants. If neccessary the specimen must be concentrated before testing (ultrafiltration i.e.).
Specimen with high or low pH, high salt or detergens concentration should be dialysed against phosphate buffered saline (PBS).