EE(Ethinylestradiol) ELISA Kit

Sample

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Method

cELISA

Intended Use

For quantitative detection of EE in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Storage

1. After opening the package, please check whether the items are complete in time.
2. Kit storage: -20°C (for long-term storage, the reagents should be stored at the temperature indicated on the label); 2-8°C (use within one week); avoid repeated freezing and thawing, and do not use after expiration.
3. When the concentrated washing solution is stored at low temperature, there will be salt precipitation. When diluting, it can be heated in a water bath to help dissolve it.
4. There may be a small amount of water-like substances in the wells of the newly opened microtiter plate, which is a normal phenomenon and will not have any impact on the experimental results.
5. All kit components have been tested for formulation and quality control to function successfully as a kit. Do not mix or substitute reagents or materials from other kits.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Detection Range

31.25-2000 pg/mL

Sensitivity

9.34 pg/mL

Standard Curve

Results of a typical standard operation of a EE ELISA Kit are listed below. This standard curve was generated at our lab for demonstration purpose only. Users shall obtain standard curve as per experiment by themselves. (N/A=not applicable)