17β-Estradiol ELISA Kit

Name: 17β-Estradiol ELISA Kit
SKU: DEIABL248
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Fecal Extracts, Urine, Tissue Culture Medi

Detection Method

cELISA

Intended Use

The DEIABL248 uses a specifically generated antibody to measure estradiol and its metabolites in urine and fecal samples. This kit is not recommended for serum, plasma, or saliva samples.

Contents of Kit

1. Coated Clear 96 Well Plates: Clear plastic microtiter plate(s) coated with goat anti-rabbit IgG. 1 plate
2. Estradiol Standard: Estradiol at 100,000 pg/mL in a special stabilizing solution. 125 μL
3. Estradiol Antibody: A rabbit polyclonal antibody speciffc for estradiol. 3 mL
4. Estradiol Conjugate: A estradiol-peroxidase conjugate in a special stabilizing solution. 3 mL
5. Assay Buffer Concentrate:
A 5× concentrate that should be diluted with deionized or distilled water. 28 mL
6. Wash Buffer Concentrate:
A 20× concentrate that should be diluted with deionized or distilled water. 30 mL
7. TMB Substrate: 11 mL
8. Stop Solution:
A 1M solution of hydrochloric acid. CAUSTIC. 5 mL
9. Plate Sealer:

Storage

All components of this kit should be stored at 4°C until the expiration date of the kit.

Precision

Intra Assay Precision
Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Estradiol concentrations were:

Inter Assay Precision
Three human samples were diluted with Assay Buffer and run in duplicates in thirteen assays run over multiple days by four operators. The mean and precision of the calculated Estradiol concentrations were:

Detection Limit

The Limit of Detection for the assay was determined in a similar manner by comparing the OD's for twenty runs for each of the zero standard and a low concentration human sample. Limit of Detection was determined as 26.5 pg/mL

Sensitivity

Sensitivity was calculated by comparing the OD's for twenty wells run for each of the B0 and standard #5. The detection limit was determined at two (2) standard deviations from the B0 along the standard curve. Sensitivity was determined as 39.6 pg/mL.

Standard Curve

Always run your own standard curve for calculation of results. Do not use this data.
Conversion Factor: 100 pg/mL of estradiol is equivalent to 367.1 pM.

Always run your own standard curves for calculation of results. Do not use this data.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Trait Negative Affect Interacts With Ovarian Hormones to Predict Risk for Emotional Eating

CLINICAL PSYCHOLOGICAL SCIENCE

Authors: Mikhail, Megan E.; Keel, Pamela K.; Burt, S. Alexandra; Sisk, Cheryl L.; Neale, Michael; Boker, Steven; Klump, Kelly L.

Abstract

Ovarian hormones significantly influence dysregulated eating in females. However, most women do not develop appreciable disordered eating, which suggests that ovarian hormones may not affect all women equally. We examined whether individual differences in trait negative affect (NA) moderate ovarian hormone-dysregulated eating associations in 446 women who provided saliva samples for hormone measurements and ratings of NA and emotional eating daily for 45 consecutive days. Women were at greatest risk for emotional eating when they had high trait NA and experienced a hormonal milieu characterized by low estradiol or high progesterone. Although effects were evident in all women, the combination of high trait NA and high progesterone was particularly risky for women with a history of clinically significant binge-eating episodes. These findings provide initial evidence that affective and hormonal risk interact to promote emotional eating and that effects may be amplified in women with clinically significant binge eating.

Sensitive Determination of 17 beta-estradiol using a Magneto Sensor Based on Magnetic Molecularly Imprinted Polymer

ELECTROANALYSIS

Authors: Nunes da Silva, Daniela; Leijoto de Oliveira, Hanna; Borges, Keyller Bastos; Pereira, Arnaldo Cesar

Abstract

The analysis of 17 beta-estradiol with high sensitivity and selectivity is extremely relevant to control the impacts that this compound can cause on health and the environment. Thus, we describe the development and application of a magneto carbon paste electrode based on magnetic molecularly imprinted polymer (MCPE-MMIP) for determination of 17 beta-estradiol. The analyte adsorbed on the MMIP was immobilized on the electrode surface by magnetic capture. The morphological and structural characterization of the obtained MMIP suggests that the material was effectively synthesized. MCPE-MMIP showed an improvement in the sensitivity for 17 beta-estradiol detection when compared to electrode configurations in the absence of this material. The optimum conditions (0.10 mol L(-1)phosphate buffer pH 7.0) were reached by differential pulse adsorptive stripping voltammetry (DPAdSV), in which the method presented linearity ranged from 0.06 to 175 mu mol L(-1)with limits of detection and quantification of 0.02 and 0.06 mu mol L-1, respectively. The proposed sensor was applied effectively in the analysis of 17 beta-estradiol in river water and raw milk samples, exhibiting excellent recovery values (between 96.20 and 104 %), which were confirmed by HPLC analysis.