Sample
Serum, plasma, tissue homogenates
Species Reactivity
Bovine
Intended Use
For the quantitative determination of bovine anti-mullerian hormone (AMH) concentrations in serum, plasma, tissue homogenates. FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
Contents of Kit
1. Assay plate, 1(96 wells)
2. Standard, 6×0.5 ml (0ng/ml, 0.5ng/ml, 1.75ng/ml, 6.25ng/ml, 37.5ng/ml, 200ng/ml)
3. Antibody, 1×6 ml
4. HRP-conjugate, 1×6 ml
5. Wash Buffer (20×concentrate), 1×15 ml
6. Substrate A, 1×7 ml
7. Substrate B, 1×7 ml
8. Stop Solution, 1×7 ml
9. Adhesive Strip (For 96 wells), 4
Storage
Unopened kit: Store at 2-8°C. Do not use the kit beyond the expiration date.
Opened kit: May be stored for up to one month at 2-8°C.
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Detection Limit
0.5 ng/ml-200 ng/ml.
Sensitivity
The minimum detectable dose of bovine AMH is typically less than 0.5 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine AMH concentration that could be differentiated from zero.
Citations
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Rey, R; Lukas-Croisier, C; et al. AMH/MIS: what we know already about the gene, the protein and its regulation. MOLECULAR AND CELLULAR ENDOCRINOLOGY 211:21-31(2003).
Cabianca, G; Rota, A; et al. Expression of AMH in female fetal intersex gonads in the bovine. ANATOMIA HISTOLOGIA EMBRYOLOGIA-JOURNAL OF VETERINARY MEDICINE SERIES C 36:24-26(2007).
COA
Certificate of Analysis
