AMH ELISA Kit

Name: AMH ELISA Kit
SKU: DEIA-Z00113V2
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

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COA

SDS

Datasheet

Sample

Serum, plasma

Species Reactivity

Human

Intended Use

The AMH ELISA is an enzyme immunoassay for the quantitative measurement of Anti-Müllerian Hormone (AMH) in serum or plasma (EDTA or lithium heparin plasma).

Storage

When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date. Opened reagents must be stored at 2 °C to 8 °C. Microtiter wells must be stored at 2 °C to 8 °C. Once the foil bag has been opened, care should be taken to close it tightly again. Opened kits retain activity for 8 weeks if stored as described above.

Detection Range

The range of the assay is between 0.062 ng/mL – 20.0 ng/mL.

Detection Limit

The detection limit of the ELISA was calculated by adding 2 standard deviations to the mean of 20 replicate analyses of the Standard 0 and was found to be 0.044 ng/mL.
The Limit of Blank (LoB) is 0.044 ng/mL.
The Limit of Detection (LoD) is 0.052 ng/mL.
The Limit of Quantification (LoQ) is 0.062 ng/mL.

General Description

Anti-Müllerian hormone also known as AMH is a protein that, in humans, is encoded by the AMH gene.It inhibits the development of the Müllerian ducts (paramesonephric ducts) in the male embryo. It has also been called Müllerian inhibiting factor (MIF), Müllerianinhibiting hormone (MIH), and Müllerian-inhibiting substance (MIS). AMH is a protein hormone structurally related to inhibin and activin, and a member of the transforming growth factor-β (TGF-β) family. It is a dimeric glycoprotein that has a molar mass of 140 kDa. AMH is secreted by Sertoli cells of the testes during embryogenesis of the fetal male. In females, it is secreted by the granulosa cells of ovarian follicles. In mammals, AMH prevents the development of the müllerian ducts into the uterus and other müllerian structures. The effect is ipsilateral, that is each testis suppresses Müllerian development only on its own side. In humans, this action takes place during the first 8 weeks of gestation. AMH is expressed by granulosa cells of the ovary during the reproductive years, and controls the formation of primary follicles by inhibiting excessive follicular recruitment by FSH.

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Customer Reviews

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Testicular function in boys with 47,XYYand relationship to phenotype

AMERICAN JOURNAL OF MEDICAL GENETICS PART C-SEMINARS IN MEDICAL GENETICS

Authors: Davis, Shanlee M.; Bloy, Luke; Roberts, Timothy P. L.; Kowal, Karen; Alston, Amanda; Tahsin, Aysha; Truxon, Alyssa; Ross, Judith L.

Abstract

An additional Y chromosome occurs in similar to 1 in 1,000 males, resulting in the karyotype 47,XYY. The phenotype includes tall stature, hypotonia, neuropsychiatric comorbidities, and an increased risk of infertility in adulthood. Little is known about testicular function in childhood and adolescence in 47,XYY. This cross-sectional study aimed to assess testicular function serum biomarkers, including total testosterone, inhibin B, and anti-mullerian hormone (AMH), in 82 boys with XYY (11.3 +/- 3.8 years) compared with 66 male controls (11.6 +/- 3.8 years). The association of testicular hormones with physical features, neuropsychological phenotype, and magnetoencephalography (MEG) was assessed with multiple linear regression models. Results indicate males with XYY have significantly lower inhibin B (median 84 pg/ml vs. 109 pg/ml, p= .004) and higher AMH (median 41 ng/ml vs. 29 ng/ml,p= .011); however, testosterone, testicular volume, and stretched penile length were not different from controls. In the exploratory analysis of relationships between hormone concentrations and phenotypic assessments, higher inhibin B concentrations were positively correlated with lower BMI and better cognitive, academic, and behavioral outcomes in the XYY group. Testosterone concentrations were positively associated with better behavioral outcomes in boys with XYY. Higher testosterone and inhibin B concentrations were also associated with shorter auditory latencies measured using magnetoencephalography (MEG) in XYY. With a few exceptions, testicular hormones were not associated with phenotypic outcomes in controls. In conclusion, there is evidence of subtle impaired testicular function in boys with XYY and a newly described relationship between measures of testicular function and some aspects of the XYY phenotype.

Increased risk of maternal and neonatal complications in hormone replacement therapy cycles in frozen embryo transfer

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY

Authors: Zong, Liping; Liu, Peihao; Zhou, Liguang; Wei, Daimin; Ding, Lingling; Qin, Yingying

Abstract

Background The endometrial preparation during frozen embryo transfer (FET) can be performed by natural cycle (NC), hormone replacement therapy (HRT) cycle and cycle with ovulation induction (OI). Whether different FET preparation protocols can affect maternal and neonatal outcomes is still inconclusive. Methods This was a retrospective cohort study that included 6886 women who delivered singleton live birth babies after 28 weeks of pregnancy underwent FET from January, 2015 to July, 2018. Women were divided into three groups according to the protocols used for endometrial preparation during FET: NC group (N = 4727), HRT group (N = 1642) and OI group (N = 517). Results After adjusting for the effect of age, body mass index (BMI), irregular menstruation, antral follicle count (AFC), endometrial thickness, the levels of testosterone, anti-Mullerian hormone (AMH), preconceptional fasting glucose (PFG), systolic and diastolic pressure et al., the HRT group had higher risk of hypertensive disorders of pregnancy (HDP) compared with the NC group (adjusted odds ratio (aOR) 2.00, 95% confidence interval (CI) 1.54-2.60). Singletons born after HRT FET were at increased risk of low birth weight (LBW) compared to NC group (aOR 1.49, 95%CI 1.09-2.06). The risks of preterm birth (PTB) in the HRT and OI group were elevated compared with the NC group (aOR 1.78, 95%CI 1.39-2.28 and aOR 1.51, 95%CI 1.02-2.23, respectively). Conclusions The HRT protocol for endometrial preparation during frozen embryo transfer of blastocysts was associated with increased risk of maternal and neonatal complications, compared to the NC and OI protocol.