Human Iocitrate Dehydrogenase 1

Product Overview

RecombinantHuman Iocitrate Dehydrogenase 1 (NADP+), Soluble was expressed inSaccharomyces cerevisiae.

Nature

Recombinant

Tag/Conjugate

Unconjugated

Format

Purified, Liquid

Concentration

Specific activity: Lot specific 30 Units/mg protein

Buffer

0.05MPotassium phosphate, pH 7.0 containing 50% glycerol

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

Antigen Description

Isocitratedehydrogenase (EC 1.1.1.42) and (EC 1.1.1.41), also known as IDH, is anenzyme that participates in the citric acid cycle. It catalyzes the thirdstep of the cycle: the oxidative decarboxylation of isocitrate, producingalpha-ketoglutarate (α-ketoglutarate) and CO2 while converting NAD+to NADH. This is a two-step process, which involves oxidation of isocitrate(a secondary alcohol) to oxalosuccinate (a ketone), followed by thedecarboxylation of the carboxyl group beta to the ketone, formingalpha-ketoglutarate. Another isoform of the enzyme catalyzes the samereaction, however this reaction is unrelated to the citric acid cycle, iscarried out in the cytosol as well as the mitochondrion and peroxisome anduses NADP+ as a cofactor instead of NAD+.

Keywords

IDH1; isocitrate dehydrogenase 1 (NADP+), soluble; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; epididymis luminal protein 216; epididymis secretory protei