General Notes
This product (Catalog No. DPABH-26279) is a rabbit-derived polyclonal antibody raised against human IDH1. This antibody exhibits cross-reactivity with IDH1 from mouse and rat. It has been affinity purified by Protein A. Isocitrate dehydrogenase (IDH) is a family of enzymes that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (2-oxoglutarate), releasing carbon dioxide and reducing either NAD⁺ to NADH or NADP⁺ to NADPH, serving as a pivotal step in cellular metabolism. This reaction occurs in the tricarboxylic acid (TCA) cycle for energy production or in biosynthetic pathways for NADPH generation. There are three main isoforms in mammals: the NAD⁺-dependent IDH3, which functions irreversibly in the mitochondrial TCA cycle to produce NADH for ATP synthesis; and the NADP⁺-dependent IDH1 and IDH2, which are reversible and support reductive carboxylation for lipid synthesis while generating NADPH for antioxidant defense. IDH3 is a heterooctameric complex composed of α, β, and γ subunits in a 4:2:2 stoichiometry localized exclusively in mitochondria, whereas IDH1 is a homodimer in the cytosol and peroxisomes, and IDH2 is a mitochondrial homodimer. The enzymes require divalent metal ions such as Mg²⁺ or Mn²⁺ to coordinate the substrate isocitrate in the active site, facilitating hydride transfer and decarboxylation.
DPABH-26279 is suitable for use in WB 1 (500-1:2000), IHC 1 (50-1:200) applications.
DPABH-26279 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
COA
Publication 
Loading ......
