Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The IDH1 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
IDH1; isocitrate dehydrogenase 1 (NADP+), soluble; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; epididymis luminal protein 216; epididymis secretory protein Li 26; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Kato, Y; et al. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas. BRAIN TUMOR PATHOLOGY 32:3-11(2015).
Cryan, JB; Haidar, S; et al. Clinical multiplexed exome sequencing distinguishes adult oligodendroglial neoplasms from astrocytic and mixed lineage gliomas. ONCOTARGET 5:8083-8092(2014).
COA
Certificate of Analysis
