Recombinant Dengue Type-4 Envelope contains a common antigen for Dengue type 1, 2 and 3 and contains a 6-His fusion partner, was expressed in E. coli. Recombinant Dengue Type-4 Envelope can reacts strongly with Dengue IgG, and can reacts with Dengue IgM i
> 95% pure (10% PAGE (Coomassie)). Proprietary chromatographic technique
2-8°C short term, -20°C long term
Dengue virus (DENV) in one of four serotypes is the cause of dengue fever. It is a mosquito-borne single positive-stranded RNA virus of the family Flaviviridae; genus Flavivirus. All four serotypes can cause the full spectrum of disease. Its genome is about 11000 bases that codes for three structural proteins, capsid protein C, membrane protein M, envelope protein E; seven nonstructural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5; and short non-coding regions on both the 5' and 3' ends. Further classification of each serotype into genotypes often relates to the region where particular strains are commonly found or were first found.
Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as an homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes.
DENV; Dengue Virus Envelope Protein; DENV Envelope Protein; Dengue virus