Rat IgA ELISA Kit

Name: Rat IgA ELISA Kit
SKU: DEIA674
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma

Species Reactivity

Rat

Detection Method

Sandwich-ELISA

Intended Use

This enzyme linked immunosorbent assay (ELISA) is for the detection of Rat IgA in serum or plasma. Due to idiotypic and allotypic variations, this product may not accurately quantitate hybridomas, monoclonals or myelomas.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Rat IgA Pre-Coated 96-well Strip Plate	1 each	2-8°C
2	Rat IgA Standard, 1000 ng/vial	2 each	2-8°C
3	Rat IgA Detection Antibody	12 ml	2-8°C
4	10× Dilution Buffer B	25 ml	2-8°C
5	HRP Solution A	12 ml	2-8°C
6	TMB Substrate	12 ml	2-8°C
7	Stop Solution	12 ml	2-8°C
8	20× Wash Buffer	50 ml	2-8°C
9	Sealing Tape	6 sheets

Storage

Stored at 2-8°C for 6 months from date of receipt.

General Description

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgG, and IgA. The rat IgG antibody has multiple functions including opsonization, complement function, and antibody-dependent cell mediated cytotoxicity. Rat IgG has been further categorized into subclasses: IgG1, IgG2a, IgG2b, and IgG2c. Functionally, Rat IgG1 and IgG2a have been shown to bind complement. This is in contrast to mice where only IgG2a binds complement (Overy 1966, Medgyesi 1974). In rats, as with other mammals, IgA functions primarily in mucosal immunity (Nash 1969). Rat IgM functions primarily as a naïve B-cell receptor and in complement functions. Concentrations of Rat IgA in serum are typically shown to be in the range of 0.01 to 0.2 mg/ml.

Standard Curve

This typical standard curve was generated using the Rat IgA ELISA Kit Protocol. This standard curve is for demonstration only. A standard curve must be generated for each assay.