Mouse IgA ELISA Kit

Name: Mouse IgA ELISA Kit
SKU: DEIA651
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Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum or plasma

Species Reactivity

Mouse

Detection Method

Sandwich-ELISA

Intended Use

This enzyme linked immunosorbent assay (ELISA) is for the detection of Mouse IgA in serum or plasma. Other biological fluids that contain Mouse IgA, such as milk, feces and saliva, may be suitable samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Mouse IgA Pre-Coated 96-well Strip Plate	1 each	2-8°C
2	Mouse IgA Standard, 1000 ng/vial	2 each	2-8°C
3	Mouse IgA Detection Antibody	12 ml	2-8°C
4	10× Dilution Buffer B	25 ml	2-8°C
5	HRP Solution A	12 ml	2-8°C
6	TMB Substrate	12 ml	2-8°C
7	Stop Solution	12 ml	2-8°C
8	20× Wash Buffer	50 ml	2-8°C
9	Sealing Tape	6 sheets

Storage

Stored at 2-8°C for 6 months from date of receipt.

General Description

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgG, and IgM. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. IgA occurs as a single class in mice, whereas in humans, there are two subclasses: IgA1 and IgA2. IgA is the major immunoglobulin found in secretory fluids such as saliva, tears, and colostrum. Secretory IgA is also the most abundant immuoglobulin in the respiratory and intestinal mucosa where it serves as a first line of defense against bacterial and viral invasion from the external environment. Secretory IgA is mainly formed as dimers and higher multimers that are linked via a J-chain attached near the Fctermini. Secretory IgA also contains a 70 kDa polypeptide associated with the alpha heavy chain, the secretory component (SC), which has been shown to protect IgA molecules from protease activity (Tizard, 1982). IgA is also present in the serum, but in a monomeric form where it functions mainly in the inflammatory response via its interactions with receptors specific for the IgA Fc region (FcalphaR) on effector immune cells (Snoeck, Peters, & Cox, 2006). The reported concentration of serum IgA in normal BALB/c mice is around 0.7mg/ml (Quimby & Luong Richard, 2007).

Standard Curve

This typical standard curve was generated using the Mouse IgA ELISA Kit Protocol. This standard curve is for demonstration only. A standard curve must be generated for each assay.