For the quantitative determination of Dog IgA antibody.FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
Contents of Kit
No.
Components
Size
Storage Conditions
1
Anti Dog IgA Coated 96-well Plate
12×8 wells
2-8°C
2
HRP Conjugate
11 ml
2-8°C
3
IgA Stock (lyophilized)
1
2-8°C
4
20× Wash Solution
50 ml
2-8°C
5
10× Diluent
25 ml
2-8°C
6
TMB Reagent
11 ml
2-8°C
7
Stop Solution
11 ml
2-8°C
Storage
The test kit will remain stable for six months from the date of purchase provided that the components are stored at 2°C-8°C. The microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air.
General Description
The dog IgA ELISA kit is designed for measurement of IgA in serum or plasma. The assay uses goat anti-dog IgA for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-dog IgA antibodies for detection. Both capture and detection antibodies were cross absorbed on dog IgG and IgM agarose columns, thereby ensuring specificity for IgA.
Standard Curve
A typical standard curve with optical density readings at 450 nm on the Y-axis against log10 IgA concentrations on the X-axis is shown below. This curve is for the purpose of illustration only. It should not be used to calculate unknowns. Each user should obtain his or her data and standard curve in each experiment.
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