Pig IgA ELISA Kit

Sample

Serum, Plasma, Colostrum, Milk

Species Reactivity

Pig

Detection Method

Sandwich-ELISA

Intended Use

This enzyme linked immunosorbent assay (ELISA) is for the detection of pig IgA in serum, plasma, colostrum and milk. Other biological samples, such as saliva, tears, nasal and intestinal secretions, may be suitable samples.

Contents of Kit

Storage

Stored at 2-8°C for 6 months from date of receipt.

General Description

IgA is the major immunoglobulin found in secretory fluids such as saliva, tears, nasal secretions and intestinal secretions. IgA is the most abundant immunoglobulin in the respiratory and intestinal mucosa where it serves as a first line of defense against bacterial and viral invasion from the external environment. Secretory IgA is mainly formed as dimers and higher multimers that are linked via a J-chain linked near the Fc-termini. Secretory IgA also contains a 70 kDa polypeptide associated with the alpha heavy chain, the secretory component (SC), which has been shown to protect IgA molecules from protease activity (Tizard, 1982). IgA is also present in the serum, but in a monomeric form where it functions mainly in the inflammatory response via its interactions with receptors specific for the IgA Fc region (FcalphaR) on effector immune cells (Snoeck, Peters, & Cox, 2006). Reported levels of IgA in pig serum range from 1.32 – 1.56 mg/ml; in colostrum, levels range from 9.0 – 9.6 mg/ml; in milk, levels range from 2.8 – 4.8. (Butler, 1983).

Standard Curve

This typical standard curve was generated using the Pig IgA ELISA Kit Protocol. This standard curve is for demonstration only. A standard curve must be generated for each assay.

*Suggested standard curve points are 1000 ng/ml, 333.3, 111.1, 37, 12.3, 4.1 1.37, and 0 ng/ml. Assay Range: 1.37 – 1000 ng/ml