Chicken IgA ELISA Kit

Name: Chicken IgA ELISA Kit
SKU: DEIA627
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, or other biological fluids

Species Reactivity

Chicken

Detection Method

Sandwich-ELISA

Intended Use

This enzyme linked immunosorbent assay (ELISA) is for the detection of Chicken IgA in serum, plasma, or other biological fluids.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Chicken IgA Pre-Coated 96-well Strip Plate	1 each	2-8°C
2	Chicken IgA Standard, 1000 ng/vial	2 each	2-8°C
3	Chicken IgA Detection Antibody	12 ml	2-8°C
4	10× Dilution Buffer B	25 ml	2-8°C
5	HRP Solution A	12 ml	2-8°C
6	TMB Substrate	12 ml	2-8°C
7	Stop Solution	12 ml	2-8°C
8	20× Wash Buffer	50 ml	2-8°C
9	Sealing Tape	6 sheets

Storage

Stored at 2-8°C for 6 months from date of receipt.

General Description

Avian antibodies have been recognized for decades and offer many advantages to mammalian antibodies in research and diagnostics. The only avian species in which antibodies are highly defined and easily accessible are the chicken. In mammals, immunoglobulins are classified into five groups: IgA, IgD, IgE, IgG and IgA. Chickens possess three principle classes of immunoglobulins: IgM, IgY (IgG) and IgA (Lebacq-Verheyden, 1974). Moreover, the structures of the corresponding immunoglobulins are significantly different, namely in the number of constant domains within the heavy chains. While avian IgY (IgG) is comprised of four constant domains, mammalian IgG contains only three (Hodek, 2003). The average concentration of IgA in chicken serum is about 0.33 mg/ml and represents less than 4% of the total immunoglobulins. However, chicken IgA is the predominate immunoglobulin in chicken bile and intestinal secretions (Lebacq-Verheyden, 1974). IgA is also known to be transferred with other proteins in the oviduct into the egg white but in very low amounts.

Standard Curve

This typical standard curve was generated using the Chicken IgA ELISA Kit Protocol. This standard curve is for demonstration only. A standard curve must be generated for each assay.