Anti-RSV polyclonal antibody

The rhizome of Dryopteris crassirhizoma Nakai is an important traditional Chinese medicine listed in Chinese Pharmacopoeia for the treatment of parasitic infestation and viral diseases. In this work, ten enantiomeric pairs of new acylphloroglucinol meroterpenoids, ( +/- )-dryocrassoids A - J [( +/- )-1 - 101, along with six known acylphloroglucinols (11-16) were isolated from the rhizomes of D. crassirhizoma. Notably, these new enantiomers were the first examples of meroterpenoids constructed by acylphloroglucinols and nerolidol-type sesquiterpenes, while ( +/- )-1a/1b, ( +/- )-2a/2b, ( +/- )-4a/4b were obtained as the inseparable interconverting mixtures of keto and enol tautomers. The new structures and absolute configurations were identified by the interpretation of high resolution electron spray ionization mass spectrum (HRESIMS), nuclear magnetic resonance (NMR) spectroscopic data, and quantum chemical electronic circular dichroism (ECD) calculations. In addition, these compounds were assayed for the in vitro antiviral activities against herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), and respiratory syncytial virus (RSV). The results indicated that most of the compounds showed anti-HSV-1 and anti-RSV effects to different extent under the concentration of 100 mu M. This study provided evidences for further study and utilization of D. crassirhizoma in medicinal products.

Renal cell carcinoma (RCC) is one of the most common urologic malignant tumors. Current chemotherapy is not effective in RCC and results in some side effects. Resveratrol (RSV) has been reported to exert antitumor effects in some cancer cells; however the mechanism is not fully understood. Herein, we aimed to determine the anticancer effect of RSV on RCC and further explore the underlying molecular mechanism in this process. We found that RSV inhibited tumor cells proliferation, migration and invasion and increased apoptosis of RCC either in vivo or in vitro. RSV significantly down-regulated expressions of NLRP3 and its downstream genes. Inhibition of NLRP3 by NLRP3 small interfering RNA mimicked the effects of RSV on RCC cells. These results suggested that RSV could exert antitumor effect by depressing activity of NLRP3, and NLRP3 would be a promising clinical therapeutic strategy for RCC. (C) 2020 Published by Elsevier Inc.