Contents of Kit
1.Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96) MTP, 1 frame, the coating material is inactivated, 12
2.Cut-off serum (ready-to-use) C/O, Human serum in protein containing phosphate buffer; negative for anti-HIV Ab, HBs-Ag (Hepatitis B-Virus surface antigen) and anti-HCV Ab; preservative: < 0.1 % sodium azide; colouring: Chinaldin yellow, 2x 2 ml
3.Negative control serum (ready-to-use) NEG Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide, colouring: Lissamin green V, 2 ml
4.Positive control serum (ready-to-use) POS, Human serum in protein containing phosphate buffer; negative for anti-HIV Ab, HBs-Ag (Hepatitis B-Virus surface antigen) and anti-HCV Ab; preservative: < 0.1 % sodium azide; colouring: Amaranth O
5.Anti-human-IgM-conjugate (ready-to-use) APC Anti-human-IgM from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution, preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane, 13 ml
6.Washing solution concentrate (sufficient for 1 litre) WASH Sodium chloride solution with Tween 20, 30 mM Tris, preservative: < 0.1 % sodium azide, 33.3 ml
7.Dilution buffer DILB Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide 0.01 g/l Bromphenol blue sodium salt, 2 x 50 ml
8.Stopping solution STOP 1.2 N sodium hydroxide, 15 ml
9.Substrate (ready-to-use) pNPP Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide, (Substrate in unopened bottle may have a slightly yellow coloring. This does not reduce the quality of the product!), 13 ml
10.Quality control certificate INFO, 1
General Description
Respiratory Syncytial Virus (RSV) causes upper and lower respiratory tract infections predominantly in infants and children. Such infections are responsible for bronchitis as well as pneumonia and may be life threatening. In addition, an RSV infection may often result in middle ear inflammation. In older children and adults, an infection is usually associated with mild symptoms although the elderly may develop more sever symptoms.
RSV is a pleomorphic RNA virus with a diameter of around 150-300nm. Originally isolated from chimpanzees, the firus acquired its name from the ability to form syncytia when cell cultures are infected with the virus.
Infections are transmitted from host to host through contact with mucous membranes of the eyes, mouth, or nose. Epidemiological studies have shown that all children by the age of 2 years have already experienced an RSV infection. Immunity developed as a result of infection is poor due to the virus spreading by syncytia formation. A protective immunity is primarily due to antibody responses to the viral surface glycoproteins G and F, whereby the F protein stimulates a humoral as well as a cellular immune response.
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