5-(Perylen-3-ylethynyl)uracil Derivatives Inhibit Reproduction of Respiratory Viruses
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
Authors: Nikolayeva, Y. V.; Ulashchik, E. A.; Chekerda, E. V.; Galochkina, A. V.; Slesarchuk, N. A.; Chistov, A. A.; Nikitin, T. D.; Korshun, V. A.; Shmanai, V. V.; Ustinov, A. V.; Shtro, A. A.
Abstract
In this work, we describe the synthesis of 5-(perylen-3-ylethynyl)uridine and its ability to effectively inhibit the replication of respiratory disease pathogens in cell culture, namely: influenza A virus (IVA); type 3 parainfluenza virus (PIV-3); and human respiratory syncytial virus (RSV). Related known compounds were also analyzed: 5-(perylen-3-ylethynyl)-2 '-deoxy-uridine; 5-(perylen-3-ylethynyl)-arabino-uridine; and 1-carboxymethyl-3-pivaloyloxymethyl-5-(perylen-3-ylethynyl)uracil.
Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs
PLANT PATHOLOGY JOURNAL
Authors: Fang, Ying; Choi, Jae Young; Park, Dong Hwan; Park, Min Gu; Kim, Jun Young; Wang, Minghui; Kim, Hyun Ji; Kim, Woo Jin; Je, Yeon Ho
Abstract
RNA interference (RNAi) has attracted attention as a promising approach to control plant viruses in their insect vectors. In the present study, to suppress replication of the rice stripe virus (RSV) in its vector, Laodelphax striatellus, using RNAi, dsRNAs against L. striatellus genes that are strongly upregulated upon RSV infection were delivered through a rice leaf-mediated method. RNAi-based silencing of peroxiredoxin, cathepsin B, and cytochrome P450 resulted in significant down regulation of the NS3 gene of RSV, achieving a transcriptional reduction greater than 73.6% at a concentration of 100 ng/mu l and, possibly compromising viral replication. L. striatellus genes might play crucial roles in the transmission of RSV; transcriptional silencing of these genes could suppress viral replication in L. striatellus. These results suggest effective RNAi-based approaches for controlling RSV and provide insight into RSV-L. striatellus interactions.