Phosphatidylinositol 3,4-bisphosphate ELISA Kit

Sample

cells extracts

Intended Use

The PI(3,4)P2 Mass ELISA measures the amount of PI(3,4)P2 extracted from cells by means of a competitive ELISA.

Contents of Kit

1. PI(3,4)P2 Coated Strip-well Detection Plate: 1 plate
2. PI(3,4)P2 Standard: 10.4 μg
3. PI(3,4)P2 Detector: 20 μL
4. Secondary Detector: 300 μL
5. 10x PBS-T: 20 mL
6. Protein Stabilizer: 600 μL; 2 vials
7. TMB Solution: 12 mL
8. 1 N H2SO4 Stop Solution: 10 mL
9. Colored 96-well polypropylene U-bottom plate: 1 plate
10. Clear acetate sheet, 1 side adhesive: 3

Storage

2-8°C

Detection Range

300 to 1.23 pmol PI(3,4)P2

General Description

The assay is a competitive ELISA where the signal is inversely proportional to the amount of PI(3,4)P2 produced. This allows the user to measure the amount of PI(3,4)P2 extracted from cells by means of standard 96 well ELISA format, eliminating the need for radioactivity and thin layer chromatography. Once PI(3,4)P2 has been extracted from cellular samples, it is incubated with a PI(3,4)P2 detector, then added to the PI(3,4)P2-coated plate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect PI(3,4)P2 detector binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(3,4)P2 extracted from cells.