Phosphatidylinositol 4-phosphate ELISA Kit

Sample

cells extracts

Intended Use

The Phosphatidylinositol 4-phosphate ELISA Kit is designed to detect and quantify PI(4)P by means of a competitive ELISA format, eliminating the need for radioactivity and thin layer chromatography. The PI(4)P Mass ELISA directly detects PI(4)P over all other phosphonositides at relevant biological levels.

Contents of Kit

1. Colored Mixing Plate: 1 plate
2. Acetate Plate Seal: 2 seals
3. PI(4)P Detector: 1 vial
4. PI(4)P Detection Plate: 1 plate
5. PI(4)P (diC16) Standard: 1 vial
6. Diluent: 1 bottle with lyophilized pellet
7. 10x PBS-T Buffer: 1 bottle of 20 mL
8. Secondary Detector: 1 vial of 300 μL
9. TMB Substrate: 1 bottle of 12 mL
10. 1 N H2SO4 Stop Solution: 1 bottle of 10 mL

Storage

The kit comes in two parts with different storage requirements. Upon receipt store Kit Part 1 at 4°C and Kit Part 2 at -20°C. Store prepared reagents as indicated in the protocol.

Detection Range

50-0.7 pmol PI(4)P

General Description

The PI(4)P Mass assay is a competitive ELISA in which the signal is inversely proportional to the amount of PI(4)P measured. The PI(4)P extractions are added to the colored mixing plate with PI(4)P Grip. This mixture is then transferred to the PI(4)P Detection Plate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the amount of PI(4)P Grip binding to the plate. The assay is sensitive to 0.7 pmol PI(4)P.

Standard Curve