Phosphatidylinositol 4-kinase ELISA Kit

Intended Use

The Phosphatidylinositol 4-kinase ELISA Kit has been designed to detect and quantify, the end product of a PI 4-Kinase reaction, PI(4)P. The PI 4-Kinase Activity ELISA is a competitive ELISA in which the signal is inversely proportional to the amount of PI(4)P measured.

Contents of Kit

1. Colored Mixing Plate: 96-well/1 plate
2. Acetate Plate Seal: 3 Seals
3. PI(4)P Detector: 0.5 μg/1 vial
4. PI(4)P Detection Plate: 96-well/1 plate
5. PI(4)P Standard: 4.79 μg/1 vial
6. Diluent: Powder/1 bottle
7. PI Substrate: 500 μg/1 vial
8. 100 mM EDTA: 100 μL/1 vial
9. ER Buffer: Powder/1 vial
10. 10x PBS-T Buffer: 20 mL/1 bottle
11. Secondary Detector: 300 μL/1 vial
12. TMB Solution: 12 mL/1 bottle
13. 1 N H2SO4 Stop Solution: 10 mL/1 bottle

Storage

2-8°C and -20°C

General Description

Phosphatidylinositol 4-phosphate (PI(4)P) is the most abundant monophosphorylated phosphoinositide found in mammalian cells. It is produced by PtdIns 4-Kinases (PI4K) which phosphorylates the D-4 position of the inositol ring of PtdIns. PI 4-Kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. Type II class PI 4-Kinases includes PI4KIIa (PI4K2A) which is involved in many biologic processes ranging from cell growth to endo- and exocytosis. It is also a novel regulator of tumor growth and generates PtdIns4P-rich domains within the Golgi. PI4KIIb (PI4K2B) is primary cytosolic where it is recruited to the membrane and stimulates PI(4,5)P2 synthesis. Type III PI 4-Kinase, PI4KIIIa (PI4K3A), plays a role in replication of the Hepatitis C virus. These viruses use PI4KIIIa to generate PI(4)P-enriched environments increasing cellular levels of PI(4)P. PI4KIIIb (PI4KB), regulates the golgi reorganization during mitosis. This enzyme is essential for glucose-induced insulin secretion due to its capacity to regulate the release of secretary granules. All of this makes PI 4-Kinases an interesting target for a diverse set of biological functions, disease states, and infections.