Batch dependent - please inquire should you have specific requirements
Buffer
0.1 M Sodium Bicarbonate, pH 7.9-8.3
Preservative
None
Storage
≤-65°C
Antigen Description
Mumps is one of the commonly acquired viral diseases of childhood and is the most common cause of aseptic meningitis. However, its incidence in developed countries has declined dramatically since the advent of vaccination. A member of the paramyxovirus family, it is closely related to parainfluenza viruses. Its genome is ss RNA of 16 - 18 kbp, and it is an enveloped virus of helical symmetry. The single RNA molecule, in a helical nucleocapsid, is in association with the nucleoprotein (NP). M protein forms the structure which underlies the viral envelope. The HN (haemagglutination and neuraminidase protein) and the F (fusion) protein form the spikes present on the lipid bilayer envelope. Only one serotype of the virus exists. There is significant antigenic cross-reaction with other members of the paramyxovirus genus.
Keywords
Mumps;MuV;Mumps virus
Citations
Publication ()
Have you cited DAGA-501 in a publication? Let us know and earn a reward for your research.
Background
Mumps virus (MuV) is a single-stranded RNA virus that belongs to the genus Rubulavirus within the family Paramyxoviridae. Its genetic material is a single-stranded RNA molecule that ranges from 16 to 18 kilobase pairs in length. The virus is enveloped and exhibits helical symmetry. Within the helical nucleocapsid, the RNA molecule is associated with the nucleoprotein (NP). The structure underlying the viral envelope is formed by the M protein. The lipid bilayer envelope features spikes composed of two key proteins: the HN protein (haemagglutination and neuraminidase) and the F protein (fusion).
Figure 1. Schematic diagram of mumps virus. (Source: Wu, H. et al., 2021)
MuV is the causative agent of mumps, a well-known childhood disease, often recognized by the swelling of the parotid glands. This characteristic swelling gives infected individuals a "chipmunk-like" appearance. In addition, mumps can cause a range of symptoms, including fever, headaches, muscle aches, fatigue, and loss of appetite. In some cases, mumps can lead to complications, such as orchitis, oophoritis, meningitis, and pancreatitis. MuV antigen refers to the antigenic component derived from MuV. The antigen is a key ingredient used in the development and production of mumps vaccines. It is responsible for stimulating the immune system to recognize and mount a protective response against the MuV. By introducing the antigen into the body through vaccination, the immune system generates a specific immune response, including the production of antibodies that can recognize and neutralize the MuV. The development and utilization of MuV antigens in vaccines have significantly contributed to controlling and preventing mumps outbreaks worldwide. Vaccination programs have been instrumental in reducing the incidence of mumps and protecting individuals, particularly children, from the disease.
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
References
Roles of Serine and Threonine Residues of Mumps Virus P Protein in Viral Transcription and Replication
Mumps virus (MuV), a paramyxovirus containing a negative-sense nonsegmented RNA genome, is a human pathogen that causes an acute infection with symptoms ranging from parotitis to mild meningitis and severe encephalitis. Vaccination against mumps virus has been effective in reducing mumps cases. However, recently large outbreaks have occurred in vaccinated populations. There is no anti-MuV drug. Understanding replication of MuV may lead to novel antiviral strategies. MuV RNA-dependent RNA polymerase minimally consists of the phosphoprotein (P) and the large protein (L). The P protein is heavily phosphorylated. To investigate the roles of serine (S) and threonine (T) residues of P in viral RNA transcription and replication, P was subjected to mass spectrometry and mutational analysis. P, a 392-amino acid residue protein, has 64 S and T residues. We have found that mutating nine S/T residues significantly reduced and mutating residue T at 101 to A (T101A) significantly enhanced activity in a minigenome system. A recombinant virus containing the P-T101A mutation (rMuV-P-T101A) was recovered and analyzed. rMuV-P-T101A grew to higher titers and had increased protein expression at early time points. Together, these results suggest that phosphorylation of MuV-P-T101 plays a negative role in viral RNA synthesis. This is the first time that the P protein of a paramyxovirus has been systematically analyzed for S/T residues that are critical for viral RNA synthesis.
Critical factors for the replication of mumps virus in primary chicken embryo fibroblasts defined by the use of design of experiments (DoE)
Live attenuated vaccines against mumps virus (MuV) have been traditionally produced by passaging the virus in the embryonated chicken eggs or primary chicken embryo fibroblasts (CEFs). Virus propagation on these cell substrates enables successful virus attenuation and retains it sufficiently antigenic to induce lasting protective immunity in humans. The aim of this study was to identify critical factors for MuV replication in primary CEFs grown on a small-scale level in order to explore possibilities for improvements in the virus replication and yield. The effect of differently prepared cells, culturing conditions, and infection conditions on virus yield was estimated by employing statistical design of experiments (DoE) methodology. Our results show that the preparation of primary CEFs and the way of their infection substantially impact virus yield and are critical for efficient MuV replication. These process parameters should be considered in further process optimization. We also demonstrate the applicability of DoE in optimization of virus replication as a crucial step in obtaining high virus yields.
Molecular biology, pathogenesis and pathology of mumps virus
Mumps is caused by the mumps virus (MuV), a member of the Paramyxoviridae family of enveloped, non-segmented, negative-sense RNA viruses. Mumps is characterized by painful inflammatory symptoms, such as parotitis and orchitis. The virus is highly neurotropic, with laboratory evidence of central nervous system (CNS) infection in approximately half of cases. Symptomatic CNS infection occurs less frequently; nonetheless, prior to the introduction of routine vaccination, MuV was a leading cause of aseptic meningitis and viral encephalitis in many developed countries. Despite being one of the oldest recognized diseases, with a worldwide distribution, surprisingly little attention has been given to its study. Cases of aseptic meningitis associated with some vaccine strains and a global resurgence of cases, including in highly vaccinated populations, has renewed interest in the virus, particularly in its pathogenesis and the need for development of clinically relevant models of disease. In this review we summarize the current state of knowledge on the virus, its pathogenesis and its clinical and pathological outcomes.
Current Status of Mumps Virus Infection: Epidemiology, Pathogenesis, and Vaccine
International Journal of Environmental Research and Public Health
Mumps is an important childhood infectious disease caused by mumps virus (MuV). We reviewed the epidemiology, pathogenesis, and vaccine development of mumps. Previous studies were identified using the key words "mumps" and "epidemiology", "pathogenesis" or "vaccine" in MEDLINE, PubMed, Embase, Web of Science, and Google Scholar. We excluded the articles that were not published in the English language, manuscripts without abstracts, and opinion articles from the review. The number of cases caused by MuV decreased steeply after the introduction of the mumps vaccine worldwide. In recent years, a global resurgence of mumps cases in developed countries and cases of aseptic meningitis caused by some mumps vaccine strains have renewed the importance of MuV infection worldwide. The performance of mumps vaccination has become an important issue for controlling mumps infections. Vaccine development and routine vaccination are still effective measures to globally reduce the incidence of mumps infections. During outbreaks, a third of MMR vaccine is recommended for groups of persons determined by public authorities.
COA
Loading ......
Publication ()
Figure 1. Schematic diagram of mumps virus.
