Mumps Virus IgM ELISA Kit

Sample

Serum, plasma or cerebrospinal fluid

Species Reactivity

Human

Detection Method

iELISA

Intended Use

ELISA Mumps Virus IgM are quantitative and qualitative tests for detection of human antibodies in serum, plasma or cerebrospinal fluid against Mumps Virus. For sale in the U.S. for Research Use Only. Not for use in diagnostic procedures.

Contents of Kit

1. Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96), 1 frame, the coating material is inactivated. 12
2. Standard serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, anti-HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1 % sodium azide, coloring: Amaranth O. 2 × 2 ml.
3. Negative control serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV, anti-HBs (Hepatitis BVirus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide coloring: Lissamin green V. 2 ml.
4. Anti-human-IgM-conjugate (ready-to-use), Anti-human-IgM from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane. 13 ml.
5. Washing solution concentrate (sufficient for 1 litre), Sodium chloride solution with Tween 20, 30 mM Tris preservative: < 0.1 % sodium azide. 33.3 ml
6. Dilution buffer, Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide, 0.01 g/l Bromphenol blue sodium salt. 2 × 50 ml
7. Stopping solution, 1.2 N sodium hydroxide. 15 ml
8. Substrate (ready-to-use), Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide. (Substrate in unopened bottle may have a slightly yellow color. This does not reduce the quality of the product!) 13 ml.

Storage

Reagent	Storage	Stability
microtiter strips (antigen)	after opening at 2-8°C in closed aluminum bag with desiccant. Strips which are not used must be stored in the press-seal bag of aluminum compound foil under dry and airtight conditions!	4 weeks
control sera / standard sera	after opening at 2-8°C	until expiry date; 24 months from date of production
conjugate	ready-to-use solution, at 2-8°C, Avoid contamination (sterile tips!)	until expiry date 28 months from date of production
washing solution	concentrate after opening at 2-8°C; working dilution at 2-8°C; working dilution at room temperature. Bottles used for the working dilution should be cleaned regularly, discard cloudy solutions.	until expiry date; 2 weeks; 1 week
dilution buffer	after opening at 2-8°C, Discard cloudy solutions!; unopened	24 months; until expiry date; 36 months from date of production
substrate	ready-to-use solution at 2-8°C, protected from light! Avoid contamination (sterile tips!) Discard when solution turns yellow (extinction against distilled water > 0.25).	until expiry date 24 months from date of production
stopping solution	after opening at room temperature	until expiry date

General Description

Parotitis (Mumps) as well as Measles, Parainfluenza and RS-viruses belong to the family of paramyxoviridae. Parotitis viruses are globally spread; humans are the only natural host. The virus is spread by droplet infection and enters the body through the upper respiratory tract. Parotitis Viruses are less contagious (40 %) than Measles and Varicella-Zoster Viruses. The highest number of incidents is observed in industrial countries among children at the age of 5-9.
The clinical picture of parotitis (parotitis epidemica, mumps) was first described by Hippocrates. Symptoms are one-sided or bilateral parotitis. Parotitis infections are relatively mild during childhood but can lead to complications in adults. Single as well as combination vaccines, like measles-parotitis or measles-parotitis-rubella, are available. Live vaccines (attenuated virus strains) provide long-lasting immunity.
Besides clinical diagnosis of Parotitis Virus infection with significant swellings, diagnosis is also performed with serological methods especially when main symptoms are missing.
Since introduction of protective vaccination, interest in laboratory diagnostics has grown.
Detection of antibodies in serum is possible with ELISAs, CFT and HHT. Immunoglobulin specific ELISAs are adapted for routine diagnostics. Direct detection of Parotitis Viruses e.g. by PCR is a complementary method.