Mumps virus (Parotitis) IgG ELISA

Name: Mumps virus (Parotitis) IgG ELISA
SKU: DEIA363
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Human Serum or Plasma

Species Reactivity

Human

Detection Method

ELISA

Intended Use

Enzyme immunoassay for the qualitative determination of IgG class antibodies against Mumps virus in human serum or plasma (citrate, heparin).

Contents of Kit

1. Microtiter Plate
2. Enzyme Conjugate
3. Positive Control
4. Negative Control
5. Cut-off Control
6. Sample Diluent
7. TMB Substrate Solution
8. Wash Buffer (20x conc)
9. Stop Solution
10. Cover foil
11. Instruction for use (IFU)

Storage

Store the kit at 2 - 8°C. The opened reagents are stable up to the expiry date stated on the label when stored at 2 - 8°C.

Performance Characteristics

The results refer to the groups of samples investigated; these are not guaranteed specifications.
The diagnostic specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte. It is 95.83% (95% confidence interval: 85.75% - 99.49%).

Precision

Sensitivity

The diagnostic sensitivity is defined as the probability of the assay of scoring positive in the presence of the specific analyte. It is 93.55% (95% confidence interval: 89.41% - 96.43%).

General Description

Mumps viruses are RNA viruses of the family Paramyxoviridae. The virions are spherical particles of 150-250 nm in diameter consisting of a ribonucleoprotein with helical symmetry and enveloped by matrix protein and a lipid bilayer which contains two spikeline structures: viral hemagglutinin (H) and viral neuraminidase (N). Mumps virus involves primarily the parotid and related salivary glands; however infection can lead to CNS disease and accumulation of the virus in CSF. Mumps (Epidemic Parotitis) is an acute contagious viral disease mostly occuring in children. Nearly 50% of all infections are subclinical. The highest incidence of clinical manifestations is found in the age group of 4 to 15 years. Secondary infections are rare because of long lasting immunity. 10 to 35% of mumps cases develop orchitis which occurs nearly always after puberty. The process is mostly unilateral and the prognosis usually good. Mumps virus has been one of the most important causes of viral CNS disease (meningitis and encephalitis) in USA; vaccine administration has greatly reduced its incidence.
Infection or presence of pathogen may be identified by: PCR & Serology: e.g. ELISA.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Reagents for Mumps Assay Development and Vaccine Research

QA & Reviews

Q & A

Ask a Question

Q: Could you please let me know if the plates are coated with viral lysate or a recombinant antigen?

A: Coating with viral lysate.

Q: Could you please let me know which antigen are coated?

A: Contains antigens against the Mumps strain Enders.

Customer Reviews

References

Investigation of mumps vaccine failures in Minsk, Belarus, 2001-2003

VACCINE

Authors: Atrasheuskaya, Alena V.; Blatun, Elena M.; Kulak, Michail V.; Atrasheuskaya, Alina; Karpov, Igor A.; Rubin, Steven; Ignatyev, George M.

Abstract

The purpose of this study was to investigate mumps vaccine failures (VF) in a highly vaccinated population of Minsk, Belarus, and to investigate a possible role for virus strain-specific immunity. During our 3-year study period, 22 adults were admitted to the Infectious Diseases Hospital in Minsk with a diagnosis of mumps. A genotype HI mumps virus (MuV) strain was identified in all patients. Of 15 patients from whom the paired sera were collected, 9 were confirmed to have been previously vaccinated. Serological examinations indicated primary VF in seven of these cases and secondary VF in two. Despite almost all vaccinated patients possessing MuV specific IgG, few possessed neutralizing antibody to the vaccine strain and titers were nominal. Importantly, none of the sera were able to neutralize a genotype H MuV strain. Our results demonstrate the importance of assaying for neutralizing antibody and support the assertion that antigenic differences between wild type and vaccine MuV strains may play a role in cases of breakthrough infection in vaccinees. @ 2007 Elsevier Ltd. All rights reserved.

Clustering-Based Weighted Extreme Learning Machine for Classification in Drug Discovery Process

NEURAL INFORMATION PROCESSING, ICONIP 2016, PT I

Authors: Kudisthalert, Wasu; Pasupa, Kitsuchart

Abstract

Extreme Learning Machine (ELM) is a universal approximation method that is extremely fast and easy to implement, but the weights of the model are normally randomly selected so they can lead to poor prediction performance. In this work, we applied Weighted Similarity Extreme Learning Machine in combination with Jaccard/Tanimoto (WELM-JT) and cluster analysis (namely, k-means clustering and Support Vector Clustering) on similarity and distance measures (i.e., Jaccard/Tanimoto and Euclidean) in order to predict which compounds with not-so-different chemical structures have an activity for treating a certain symptom or disease. The proposed method was experimented on one of the most challenging datasets named Maximum Unbiased Validation (MUV) dataset with 4 different types of fingerprints (i.e. ECFP_4, ECFP_6, FCFP_4 and FCFP_6). The experimental results show that WELM-JT in combination with k-means-ED gave the best performance. It retrieved the highest number of active molecules and used the lowest number of nodes. Meanwhile, WELM-JT with k-means-JT and ECFP_6 encoding proved to be a robust contender for most of the activity classes.