Mouse Anti-PEG IgG ELISA Kit

Sample

Serum

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

This kit is for research use only. Under no circumstances should it be used for therapeutic or diagnostic applications.

Contents of Kit

1. PEG-BSA coated plate (12 × 8-wells). Store at 4°C
2. Anti-IgG HRP Stock. Store at 4°C
3. Anti-PEG Stock. Store at 4°C
4. 20× HRP PEG Wash, 50 ml
5. HRP PEG Diluent, 50 ml
6. TMB, 12 ml
7. Stop Solution, 7 ml

Storage

All components should be stored at 4°C. The microtiter plate should be kept in a sealed bag with desiccant. Kits will remain stable for 12 months from the date of purchase provided that the components are stored as described.

General Description

Attachment of polyethylene glycol (PEG) chains to therapeutic biologic agents, a process referred to as PEGylation, prolongs the circulating half-life of the modified protein by slowing proteolytic degradation and by masking it from the immune system. However, it has been reported that repeated injections of PEGylated proteins can induce anti-PEG antibodies that increase the rate of clearance and decrease drug efficacy (accelerated blood clearance, or ABC phenomenon). To aid research in this important area, we have developed a Mouse anti-PEG IgG ELISA kit.

Standard Curve

1. Using curve fitting software, construct a standard curve by plotting absorbance values of the standards versus log10 of the concentration.
2. Fit the standard curve to a four-parameter logistic regression (4PL) equation (x axis = log10 concentration) and determine the concentration of the samples from the standard curve (remember to derive the antilog).
3. Multiply the derived concentration by the dilution factor to determine the actual concentration in the samples.
4. If the A450 values of samples fall outside the standard curve, samples should be diluted appropriately and re-tested.