Rat anti-PEG IgM ELISA Kit

Sample

serum, plasma

Species Reactivity

Rat

Detection Method

Indirect-ELISA

Intended Use

This assay primarily detects antibodies directed against the polyoxyethylene backbone of PEG.

Contents of Kit

No.	Components	Size	Storage Conditions
1	PEG-BSA Coated Plate	12×8 wells	-20°C
2	Anti-Rat IgM HRP Stock (lyophilized)	1	-20°C
3	Reference Stock (lyophilized)	1	-20°C
4	20× HRP PEG Wash	50 ml	2-8°C
5	HRP PEG Diluent	50 ml	2-8°C
6	TMB	11 ml	2-8°C
7	Stop Solution	11 ml	2-8°C

Storage

The reference stock, HRP conjugate and the PEG-BSA coated plate should be stored at -20°C. All remaining kit components should be stored at 2-8°C. The microtiter plate should be kept in a sealed bag with desiccant. Kits will remain stable for six months from the date of purchase provided that the components are stored as described.

General Description

The attachment of polyethylene glycol chains to therapeutic biologic agents, a process referred to as PEGylation, prolongs the circulating half-life of the modified protein by slowing proteolytic degradation and by masking it from the immune system. However, repeated injections of PEGylated proteins can induce anti-PEG antibodies that increase the rate of clearance and thereby decrease efficacy (accelerated blood clearance, or ABC, phenomenon). In order to aid research, we have developed a rat anti-PEG IgM ELISA.

Standard Curve

A typical standard curve with optical density readings at 450nm on the Y-axis against anti-PEG IgM concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns.