Intended Use
This kit is for research use only. Under no circumstances should it be used for therapeutic or diagnostic applications.
Contents of Kit
1. PEG-BSA coated plate (12 x 8-wells). Store at 4°C
2. Anti-IgM HRP Stock. Store at 4°C
3. Anti-PEG Stock. Store at 4°C
4. 20× HRP PEG Wash, 50 ml
5. HRP PEG Diluent, 50 ml
6. TMB, 12 ml
7. Stop Solution, 7 ml
Storage
All components should be stored at 4°C. The microtiter plate should be kept in a sealed bag with desiccant. Kits will remain stable for 12 months from the date of purchase provided that the components are stored as described.
General Description
Attachment of polyethylene glycol (PEG) chains to therapeutic biologic agents, a process referred to as PEGylation, prolongs the circulating half-life of the modified protein by slowing proteolytic degradation and by masking it from the immune system. However, it has been reported that repeated injections of PEGylated proteins can induce anti-PEG antibodies that increase the rate of clearance and decrease drug efficacy (accelerated blood clearance, or ABC phenomenon). To aid research in this important area, we have developed a Mouse anti-PEG IgM ELISA kit.
Standard Curve
A typical standard curve is shown below. This curve is for the purpose of illustration only. A standard curve must be run with each experiment.
Citations
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Q & A
Q: Is there a blocking reagent already present in each well?
A: The plates are pre-blocked during coating, and the diluent also contains blocking agents, so no additional blocking step is required.
Customer Reviews
Li, JH; Yu, XZ; et al. A biotin-streptavidin-biotin bridge dramatically enhances cell fusion. ONCOLOGY LETTERS 8:198-202(2014).
Pochechueva, T; Chinarev, A; et al. PEGylation of microbead surfaces reduces unspecific antibody binding in glycan-based suspension array. JOURNAL OF IMMUNOLOGICAL METHODS 412:42-52(2014).
Immunoassays for Process-related Impurities 
Certificate of Analysis
![Anti-Polyethylene glycol monoclonal antibody, clone RGI-D-59d [Biotin] (DMABT-Z60095)](http://img2.creative-diagnostics.com/productimages-1/Anti-Polyethylene glycol monoclonal antibody, clone RGI-D-59d [Biotin].jpg)
