Rat Anti-PEG IgG ELISA Kit

Sample

serum, plasma

Species Reactivity

Rat

Detection Method

iELISA

Intended Use

This kit is intended for research use only and is designed for the detection of anti-PEG IgG antibodies in rat serum and plasma samples. It is not intended for diagnostic or therapeutic use.

Contents of Kit

1. PEG-BSA coated plate (12 × 8-wells). Store at 2–8°C
2. Anti-IgG HRP Stock. Store at 2–8°C
3. Anti-PEG Stock. Store at 2–8°C
4. 20× HRP PEG Wash, 50 ml
5. HRP PEG Diluent, 50 ml
6. TMB, 12 ml
7. Stop Solution, 7 ml

Storage

All components should be stored at 2–8°C. The microtiter plate should be kept in a sealed bag with desiccant. The kit is stable for 12 months from the manufacturing date when stored as instructed.

General Description

Attachment of polyethylene glycol (PEG) chains to therapeutic biologic agents, a process referred to as PEGylation, prolongs the circulating half-life of the modified protein by slowing proteolytic degradation and by masking it from the immune system. However, it has been reported that repeated injections of PEGylated proteins can induce anti-PEG antibodies that increase the rate of clearance and decrease drug efficacy (accelerated blood clearance, or ABC phenomenon). To aid research in this important area, we have developed a Rat anti-PEG IgG ELISA kit.

Standard Curve

A typical standard curve is shown below. This curve is for the purpose of illustration only. A standard curve must be run with each experiment.