FKHR ELISA Kit (DEIA-XYA663)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cultured cells
Species Reactivity
Human, Mouse
Intended Use
The FKHR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FKHR protein expression profile in cells. The kit can be used for measuring the relative amounts of FKHR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FKHR.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-FKHR Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months

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References


Yu, HS; Liu, YJ; et al. Predisposition to Behcet's disease and VKH syndrome by genetic variants of miR-182. JOURNAL OF MOLECULAR MEDICINE-JMM 92:961-967(2014).
Shu, TT; Zhu, YX; et al. AGEs Decrease Insulin Synthesis in Pancreatic beta-Cell by Repressing Pdx-1 Protein Expression at the Post-Translational Level. PLOS ONE 6:-(2011).

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