General Notes
This product (Catalog No. DPABH-19229) is a rabbit-derived polyclonal antibody raised against human FOXO1, targeting the amino acid region 301–655 of the protein. This antibody exhibits cross-reactivity with Human FOXO1 from mouse and rat. It has been affinity purified by Protein A. FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).
DPABH-19229 is suitable for use in Western blot (WB) (1:1,000–1:8,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:1,000–1:4,000), IF/ICC (1:200–1:800), flow cytometry (FC) (0.40 μg per 10^6 cells in a 100 μl suspension) applications.
DPABH-19229 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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