FOXO1 ELISA Kit

Sample

Nuclear Lysates

Species Reactivity

Human; Mouse; Rat

Detection Method

iELISA

Intended Use

Detection and Qualitative Analysis of Activated Transcription Factors in Nuclear Lysates, Cytoplasmic Lysates and Tissue Homogenates. Research Purposes Only. Not Intended for Diagnostic or Clinical Procedures.

Contents of Kit

1. 96-Well dsDNA Oligonucleotide Coated Microplate: 12×8-Well Microstrips
2. 100× Primary Antibody: 100 μL
3. HRP-Conjugated Anti-Rabbit lgG Antibody: 12 ml
3. Nuclear Lysate Positive Control: Lyophilized
4. Wild-Type Consensus dsDNA Oligonucleotide, 10 μL
5. Mutant Consensus dsDNA Oligonucleotide, 10 μL
6. 10× Wash Buffer: 50 ml
7. 2× Binding Buffer: 12 ml
8. Primary Antibody Diluent: 12 ml
9. Nuclear Wash Buffer: 12 ml
10. Cytoplasmic Extraction Buffer: 6 ml
11. Nuclear Extraction Buffer: 6 ml
12. Ready-to-Use Substrate: 12 ml
13. Stop Solution: 12ml
14. Adhesive Plate Sealers: 2
15. Technical Manual: 1

Storage

Note: After using the kit the for the first time the storage times are indicated below. Reconstituted Nuclear Lysate Positive Control must be aliquoted and stored at -80°C.