Magic™ Anti-Salmonella Monoclonal antibody, Clone N0022332

Salmonella is the leading factor for microbial food poisoning. In this study, a facile pressure biosensor was developed for rapid and sensitive detection of Salmonella using magnetic nanobeads (MNBs) to separate target bacteria, platinum nanoparticle loaded manganese dioxide nanoflowers (Pt@MnO2 NFs) to amplify detection signal, and a thin-film piezoresistor based pressure detector to monitor pressure change. First, the capture antibodies (CAbs) modified MNBs were used to specifically separate Salmonella from sample to form MNB-CAbSalmonella complexes (magnetic bacteria). Then, the detection antibodies (DAbs) modified Pt@MnO2 NFs were used for labelling magnetic bacteria to form MNB-CAb-Salmonella-DAb-Pt@MnO2 NF complexes (nanoflower bacteria). After nanoflower bacteria were resuspended with H2O2 in a sealed centrifuge tube, H2O2 was catalyzed by Pt@MnO2 NFs to produce O-2, resulting in the increase on pressure. Finally, the pressure increase was real-timely monitored by piezoresistor based pressure detector and transferred to smartphone App via Bluetooth for analysis and determination of Salmonella. This biosensor could quantitatively detect Salmonella from 1.5 x 101 to 1.5 x 10(5) CFU/mL in 1.5 h with low detection limit of 13 CFU/mL. The Pt@MnO2 NFs with high loading capacity of platinum nanoparticles were demonstrated as dual mimic enzymatic catalyst of H2O2 to greatly enhance the sensitivity. More importantly, it is the first time to combine a thin-film piezoresistor with a smartphone App for realtime monitoring of the pressure change resulting from the mimic enzymatic catalysis of H2O2 into O-2 by the Pt@MnO2 NFs on the target bacteria to determine pathogenic bacteria in food samples.

A 5' amine group-linked haemagglutinin (HA) gene-specific probe was attached over the surface of a working electrode to develop a rapid, specific, and sensitive point of care detection assay for H1N1 (swine flu) in human respiratory nasal swabs. The probe was attached with a cysteine covered screen-printed gold electrode via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). The electrochemical assay was performed using differential pulse voltammetry with the use of the redox indicator methylene blue for the detection of different concentrations of the single-stranded viral genome. The developed genosensor showed high sensitivity for H1N1 influenza virus with a detection limit of 0.002 ng/6 mu L of viral nucleic acid in the sample. Samples were analysed by quantitative real-time Polymerase Chain Reaction as well as by conventional PCR. The genosensor showed high specificity, as no cross-reaction was observed with the hetemlogous nucleic acid of different pathogens (Salmonella typhi, Neisseria meningitides, and Streptococcus pyogenes) and human DNA, and it was specific for H1N1 with a sensitivity of similar to 49 mu A cm(-2) ng(-1). Genosensor is based on a very simple methodology that can be followed based on its easy-to-access approach. It is quick and could be used as a point-of-care test for the detection of influenza virus within 30 min.