Rabbit Anti-Ret monoclonal antibody, clone TO31-39

Mammea suriga Kosterm. is a RET species distributed in Malnad region of the Western Ghats, which is over exploited for flowers, seeds and timbers, hence the population is declined. The study was conducted to evaluate the germination potency of the seeds in both in vivo and in vitro conditions to derive a regeneration protocol for the ex situ conservation. Among the pre-treated seeds, the decoated seeds pre-treated with 0.5 mg L-1 NAA for 24 h resulted in 86 % of germination. In in vitro condition, culture of decoated seeds on MS medium supplemented with 0.5 mg L-1 NAA and 0.1 mg L-1 TDZ resulted 91.67 % of germination. Micropropagation studies showed that the interaction of 2-3 mg L-1 BAP and 0.2 mg L-1 TDZ induced multiple shoots from cotyledonary and hypocotyl explants. Mass of callus induced from the leaf explants at the concentration of 1 mg L-1 2,4-D and 3 mg L-1 BAP. Later on the photosynthetic callus, organise in to shoot buds at the concentration of 3 mg L-1 BAP and 0.2 mg L-1 TDZ. The excised micro shoots showed rhizogenesis on the media fortified with 1.5 mg L-1 IBA. The regenerants derived from both direct and indirect organogenesis exhibited similar morphological features.

Yuccafiber is a natural cellulose fiber that can be extracted from theYuccaplant leaves by retting. The physical properties of theYuccafiber are extremely sensitive to the retting conditions. This research was designed to study the effects of chemical retting on the structural and properties of this fiber. Chemical retting was done by soaking theYuccaleaf in 10 to 150 g/l sodium hydroxide concentration at 80 to 100 degrees C for 60 to 240 min. Fiber characteristics such as fineness, tenacity, functional groups, crystallinity, thermal degradation, and surface morphology were then investigated. TheYuccafibers exhibited high crystallinity (56-66%), high tenacity (36-46 cN/tex), and low linear density (3-5 tex). It was also found that the elementary fiber had a mean diameter of about 1.2 mu m and a helical structure of square-shaped spires. The thermogravimetric analysis also indicated that theYuccafiber had the thermal stability of up to 250 degrees C. Based on the findings, theYuccafiber may be suitable for various applications such as a reinforcement material in the composites applications and can be turned to yarn for textile applications.