Recombinant Rabbit Monoclonal Antibodies

Creative Diagnostics rabbit recombinant monoclonal antibodies offer:

Better specificity compared to mouse monoclonal antibodies.
High affinity--typically, rabbit mAbs have 10 – 100 times higher affinity than mouse monoclonal antibodies.
Lot-to-lot consistency due to recombinant technology.
Great Stability--rabbit IgG has extra disulfide bonds in the variable region of the heavy chain; an extra disulfide bond also exists between Vκ and Cκ. These extra disulfide bonds may result in the great stability and long shelf life of rabbit antibodies.
Animal origin-free formulations, the result of production by cell culture.

View all rabbit recombinant monoclonal antibodies

Recombinant Rabbit Monoclonal Antibody Production

Our recombinant rabbit monoclonal antibodies are developed by immunizing animals, screening for functionality, and cloning the immunogen-specific antibody genes into high-level expression vectors. The antibodies are produced on a large scale by expressing them in mammalian cells, and purifying them with protein A.

Recombinant Rabbit Monoclonal Antibodies

Antibody Validation

CD rabbit recombinant monoclonal antibodies are validated and characterized by multiple applications. This extensive validation process allows confidence in target specificity, without any need for optimization. Examples of their application are shown below.

Recombinant Rabbit Monoclonal Antibodies

Western blot analysis of extracts of HeLa cells, using β-actin antibody (CABT-L1115) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.

ICC staining MAP2K1 in Hela cells (green), using Rabbit Anti-MAP2K1 monoclonal antibody (DCABH-9421).
The nuclear counter stain is DAPI (blue).
Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.