Recombinant Rabbit Monoclonal Antibodies

Creative Diagnostics rabbit recombinant monoclonal antibodies offer:

• Better specificity compared to mouse monoclonal antibodies.
• High affinity--typically, rabbit mAbs have 10 – 100 times higher affinity than mouse monoclonal antibodies.
• Lot-to-lot consistency due to recombinant technology.
• Great Stability--rabbit IgG has extra disulfide bonds in the variable region of the heavy chain; an extra disulfide bond also exists between Vκ and Cκ. These extra disulfide bonds may result in the great stability and long shelf life of rabbit antibodies.
• Animal origin-free formulations, the result of production by cell culture.

View all rabbit recombinant monoclonal antibodies

Antibody Validation

CD rabbit recombinant monoclonal antibodies are validated and characterized by multiple applications. This extensive validation process allows confidence in target specificity, without any need for optimization. Examples of their application are shown below.

Western blot analysis of extracts of HeLa cells, using β-actin antibody (CABT-L1115) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.

Immunocytochemistry analysis of Human PBMCs stained with EGF recombinant rabbit monoclonal antibody (DCABH-2184) using Alexa Fluor® 488 goat anti-rabbit as a Secondary Antibody and DAPI to stain the nuclei ("b", blue).

Human stomach stained with Rabbit Anti-Human PDK1 monoclonal antibody (CABT-L1816).

Flow cytometric analysis of rabbit anti-TCL1A antibody (CABT-L1861) in RAMOS cells (green) compare to negative control of rabbit IgG (blue).