Anti-RET monoclonal antibody, clone 7F5D5

Context: Multiple endocrine neoplasia type 2B (MEN2B) is a rare cancer predisposition syndrome resulting from an autosomal-dominant germline mutation of the RET protooncogene. No prior studies have investigated pulmonary function in patients with MEN2B. Objective: This study characterized the pulmonary function of patients with MEN2B. Design: This is a retrospective analysis of pulmonary function tests (PFTs) and chest imaging of patients enrolled in the Natural History Study of Children and Adults with MEN2A or MEN2B at the National Institutes of Health. Results: Thirty-six patients with MEN2B (18 males, 18 females) were selected based on the availability of PFTs; 27 patients underwent at least 2 PFTs and imaging studies. Diffusion abnormalities were observed in 94% (33/35) of the patients, with 63% (22/35) having moderate to severe defects. A declining trend in diffusion capacity was seen over time, with an estimated slope of -2.9% per year (P = 0.0001). Restrictive and obstructive abnormalities were observed in 57% (20/35) and 39% (14/36), respectively. Computed tomography imaging revealed pulmonary thin-walled cavities (lung cysts) in 28% (9/32) of patients and metastatic lung disease in 34% (11/32) of patients; patients with metastatic lung lesions also tended to have thin-walled cavities (P = 0.035). Conclusions: This study characterized pulmonary function within a MEN2B cohort. Diffusion, restrictive, and obstructive abnormalities were evident, and lung cysts were present in 28% of patients. Further research is required to determine the mechanism of the atypical pulmonary features observed in this cohort.

Designing a stable and sensitive luminol-based electrochemiluminescence (ECL) analytical platform in the neutral condition has attracted a lot of attention. Here, gold-nanoparticle-functionalized cobalt-nickel phosphate three-dimensional nanoice creams (Au@Co3Ni3(PO4)(4) NICs) are successfully prepared via electrostatic interaction. Generally, cobalt-nickel phosphate nanoice creams (Co3Ni3(PO4)(4) NICs) are synthesized via a mild hydrothermal method and functionalized via polyethylenimine (PEI). Then, Au NPs are adsorbed on the surface of Co3Ni3(PO4)(4) NICs via Au-N weak interaction to fabricate Au@Co3Ni3(PO4)(4) NICs. Owing to the important roles of Au@Co3Ni3(PO4)(4) in exhibiting excellent electrocatalytic activity, as well as preventing the deposition of negatively charged oxidation product induced electrode passivation, luminol in the nanohybrids (LH-Au@Co3Ni3(PO4)(4)) gives strong and stable ECL intensity in the neutral conditions. Moreover, the ECL emission of luminol is obviously quenched based on the resonance energy transfer (RET) between luminol as donor and cytochrome c (Cyt c) as acceptor. Hence, a sensitive ECL biosensor is successfully fabricated for the quantitative determination of Cyt c in cell lysates and exhibits wide linear ranges of 1.0 x 10(-4) -0.5 x 10(-5) and 0.5 x 10(-5) -1.0 x 10(-8) M as well as a low detection limit of 2.48 nM. This novel sensing strategy will broaden the application of transition metal (Co, Ni) phosphates in bioassays.