GeneralInformation
Immunogen
Spleen cells from A.TL mice
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
A.TL mice spleen
Cell Line Description
Animals were immunized with spleen cells from A.TL mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Tested and found negative for ectromelia virus (mousepox).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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Lee, SH; Lillehoj, HS; et al. Development and characterization of mouse monoclonal antibodies reactive with chicken CD80. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES 34:273-279(2011).
Quah, BJC; Wijesundara, DK; et al. Fluorescent target array T helper assay: A multiplex flow cytometry assay to measure antigen-specific CD4(+) T cell-mediated B cell help in vivo. JOURNAL OF IMMUNOLOGICAL METHODS 387:181-190(2013).
COA
Certificate of Analysis
