GeneralInformation
Immunogen
Spleen cells from C3H.SW mice
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
C3H.SW mice spleen
Cell Line Description
Animals were immunized with spleen cells from C3H.SW mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. No cross-reactions are known. Tested and found negative for ectromelia virus (mousepox).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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Bhat, R; Rommelaere, J; et al. NK-cell-dependent killing of colon carcinoma cells is mediated by natural cytotoxicity receptors (NCRs) and stimulated by parvovirus infection of target cells. BMC CANCER 13:-(2013).
Wen, Y; Liu, W; et al. Antibody-functionalized peptidic membranes for neutralization of allogeneic skin antigen-presenting cells. ACTA BIOMATERIALIA 10:4759-4767(2014).