GeneralInformation
Immunogen
Spleen cells from CKB mice
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
CKB mice spleen
Cell Line Description
Animals were immunized with spleen cells from CKB mice. Spleen cells were fused with NS-1 myeloma cells. The antibody does not react with I-A of the b, d, f, p, q, r and s haplotypes. Tested and found negative for ectromelia virus (mousepox).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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Ballingall, KT; Nath, M; et al. Lack of evidence for an association between MHC diversity and the development of bovine neonatal pancytopenia in Holstein dairy cattle. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 141:128-132(2011).
McGehee, AM; Strijbis, K; et al. Ubiquitin-Dependent Control of Class II MHC Localization Is Dispensable for Antigen Presentation and Antibody Production. PLOS ONE 6:-(2011).
COA
Certificate of Analysis
