GeneralInformation
Immunogen
Lymphocytes from A.TL mice
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Mice were immunized with lymphocytes from A.TL mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not cross-react with other haplotypes. The reactivity pattern corresponds to the private specificity I-A2. Tested and found negative for ectromelia virus (mousepox).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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Lu, JM; Song, XJ; et al. Murine corneal stroma cells inhibit LPS-induced dendritic cell maturation partially through TGF-beta(2) secretion in vitro. MOLECULAR VISION 18:2255-2264(2012).
Yi, HF; Zuo, DM; et al. Suppression of antigen-specific CD4(+) T cell activation by SRA/CD204 through reducing the immunostimulatory capability of antigen-presenting cell. JOURNAL OF MOLECULAR MEDICINE-JMM 90:413-426(2012).
COA
Certificate of Analysis
