GeneralInformation
Immunogen
Spleen cells from C57BL/10J mice
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
C57BL/10J mice spleen
Cell Line Description
Animals were immunized with spleen cells from C57BL/10J mice. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with cells bearing the I-A of the b, k and u H-2 haplotypes. The antibody does not react with I-A of the d, f, q, r or s H-2 haplotypes. Tested and found negative for ectromelia virus (mousepox).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 5x10^4-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 10%(v/v) DMSO
Citations
Have you cited CSC-H0497 in a publication?
Let us know and earn a reward for your research.
| Product Name |
Cat. No. |
Applications |
Host Species |
Datasheet |
Price |
Add to Basket |
| Product Name |
Cat. No. |
Applications |
Host Species |
Datasheet |
Price |
Add to Basket |
Jeevan, A; Formichella, CR; et al. Guinea Pig Skin, a Model for Epidermal Cellular and Molecular Changes Induced by UVR in vivo and in vitro: Effects on Mycobacterium bovis Bacillus Calmette-Guerin Vaccination. PHOTOCHEMISTRY AND PHOTOBIOLOGY 89:189-198(2013).
Zhou, J; Gupta, M; et al. Immunity to the Vacuolar ATPase Complex Accessory Unit ATP6S1 in Patients with Malignant Melanoma. CANCER IMMUNOLOGY RESEARCH 3:59-67(2015).
COA
Certificate of Analysis
