Anti-ABCG2 monoclonal antibody (CABT-31717RH)
Rabbit Anti-ABCG2 monoclonal antibody for WB, ICC/IF, IHC, FC
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Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of ABCG2 on different lysates using anti-ABCG2 antibody at 1/1,000 dilution.
Lane 1: HepG2;
Lane 2: Human placenta
Fig2: ICC staining ABCG2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ABCG2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining ABCG2 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of 293T cells with ABCG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody
ABC transporter; ABC15; ABCG 2; ABCG2; ABCG2_HUMAN; ABCP
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Xia, CQ; Liu, N; et al. Expression, localization, and functional characteristics of breast cancer resistance protein in Caco-2 cells. DRUG METABOLISM AND DISPOSITION 33:637-643(2005).
de Wolf, CJF; Yamaguchi, H; et al. cGMP transport by vesicles from human and mouse erythrocytes. FEBS JOURNAL 274:439-450(2007).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.