Anti-ABCG2 monoclonal antibody (CABT-31717RH)

Rabbit Anti-ABCG2 monoclonal antibody for WB, ICC/IF, IHC, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
KG1005
Species Reactivity
Human, Mouse
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of ABCG2 on different lysates using anti-ABCG2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2; Lane 2: Human placenta
Fig2: ICC staining ABCG2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ABCG2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining ABCG2 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of 293T cells with ABCG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
ABC transporter; ABC15; ABCG 2; ABCG2; ABCG2_HUMAN; ABCP
Entrez Gene ID

Citations


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References


Scharenberg, C; Mannowetz, N; et al. ABCG2 is expressed in late spermatogenesis and is associated with the acrosome. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 378:302-307(2009).
Aye, ILMH; Paxton, JW; et al. Expression, localisation and activity of ATP binding cassette (ABC) family of drug transporters in human amnion membranes. PLACENTA 28:868-877(2007).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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