Tetanus toxoid IgG Antibody ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

This product is used for the qualitative in vitro detection of tetanus toxoid IgG antibodies in human serum/plasma or whole blood samples.
It is suitable for detecting whether the anti-tetanus toxoid IgG antibody level in normal or susceptible people has reached the full protective titer (0.1 IU/ml) and whether the anti-tetanus toxoid IgG antibody level has reached the successful level of immunity after vaccination with a vaccine containing tetanus toxoid components. Standard (0.1 IU/ml).

Contents of Kit

1. Pre-coated plate: 12T×8, purified tetanus toxoid antigen
2. Mouse anti-human IgG monoclonal antibody enzyme label: 10ml × 1 bottle, HRP enzyme-labeled mouse anti-human IgG monoclonal antibody, containing stabilizer and biological preservatives
3. Concentrated washing solution (20×): 30ml×1 bottle, 20 times concentrated phosphate buffer, containing NaCl, Tween-20
4. Sample diluent: 50ml×1 bottle, sodium citrate buffer, stabilizer, etc.
5. Negative control substance: 0.5ml×1 bottle, inactivated human anti-tetanus toxoid IgG negative serum, stabilizer
6. Positive control substance: 0.5ml×1 bottle, inactivated human anti-tetanus toxoid IgG positive serum, stabilizer
7. Substrate solution A: 5.0ml×1 bottle, the main component is carbamide peroxide
8. Substrate solution B: 5.0ml×1 bottle, main ingredient is TMB
9. Stop solution: 5.0ml×1 bottle, 0.5M H2SO4 solution
10. Ziplock bag: 1 serving
11. Sealing film: 3 sheets

Storage

1. Store refrigerated at 2~8°C and away from light. Freezing is prohibited. The validity period is 12 months.
2. Unused pre-coated boards should be immediately put into a ziplock bag with desiccant and sealed, and the storage time should not exceed one week in an environment of 2~8°C.
3. Any unused reagents of other components should be capped immediately and stored in an environment of 2~8°C. The storage time should not exceed one week.
4. Please refer to the product label for the production date and expiration date of the product.

Precision

CV%＜15%

General Description

Tetanus is a strictly anaerobic bacillus that can form spores. Tetanus is an infectious bacterial disease caused by Clostridium tetani. Under anaerobic conditions (such as a contaminated necrotic wound), this ubiquitous bacilli produces a highly toxic neurotoxin called tetanospasm toxin. Tetanus spasmotoxin blocks inhibitory neurotransmitters in the central nervous system, causing muscle rigidity and spasms that are typical of tetanus.
The incubation period of tetanus usually ranges from 3 to 21 days (median 7 days, range 0 to >60 days). In most cases, neonatal tetanus can develop between 3 and 14 days after birth. In more than 80% of cases, tetanus presents as a systemic ankylosing disease. Typical features include early facial muscle spasm (trismus and snicker), followed by dorsal muscle spasm (opistotonus) and sudden generalized tonic epilepsy (tetanus spasm). Glottic spasm can induce sudden death. In neonatal tetanus, generalized cramps are often preceded by an inability to suck or feed and excessive crying. The case fatality rate of tetanus ranges from 10% to 70%, depending on treatment, age and general health of the patient. If elderly and infant patients do not receive hospitalization and intensive treatment, the mortality rate can reach almost 100%. In hospitals with the best medical conditions, the mortality rate can be reduced to 10% to 20%.
Tetanus can occur at any age and has obvious seasonality, with its peak occurring in mid-summer or the wet season. Even with modern intensive care conditions, the case fatality rate is very high. The vast majority of tetanus cases are related to childbirth, mostly in developing countries, and are mainly seen in newborns and pregnant women with unclean delivery and poor postpartum hygiene. Tetanus following injury in children and adults is also a serious public health problem.
Laboratory methods for detecting tetanus toxoid IgG antibodies mainly include indirect hemagglutination test, toxin neutralizing antibody test, enzyme-linked immunoassay (ELISA), etc.