This kit can be used for quantitative measurement of IgM in sheep serum or plasma.
Contents of Kit
No.
Components
Size
Storage Conditions
1
Anti Sheep IgM Coated 96-well Plate
12×8 wells
4°C
2
HRP Conjugate Reagent
11 ml
4°C
3
Sheep IgM Stock (lyophilized)
1
-20°C
4
20× Wash Solution
50 ml
4°C
5
Diluent
50 ml
4°C
6
TMB Reagent (One-Step)
11 ml
4°C
7
Stop Solution (1N HCl)
11 ml
4°C
Storage
The kit will remain stable for six months from the date of purchase provided that the components are stored appropriately. Store the IgM stock vials at or below -20°C. Store the remaining components in the refrigerator at 4°C. The microtiter plate should always be kept in a sealed bag with desiccant to minimize exposure to damp air.
Standard Curve
A typical standard curve with optical density readings at 450 nm on the Y-axis against IgM concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated in each experiment.
Citations
Publication ()
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