Sheep IgM ELISA Kit

Sample

Serum and plasma

Species Reactivity

Sheep

Detection Method

Sandwich-ELISA

Intended Use

This kit can be used for quantitative measurement of IgM in sheep serum or plasma.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Anti Sheep IgM Coated 96-well Plate	12×8 wells	4°C
2	HRP Conjugate Reagent	11 ml	4°C
3	Sheep IgM Stock (lyophilized)	1	-20°C
4	20× Wash Solution	50 ml	4°C
5	Diluent	50 ml	4°C
6	TMB Reagent (One-Step)	11 ml	4°C
7	Stop Solution (1N HCl)	11 ml	4°C

Storage

The kit will remain stable for six months from the date of purchase provided that the components are stored appropriately. Store the IgM stock vials at or below -20°C. Store the remaining components in the refrigerator at 4°C. The microtiter plate should always be kept in a sealed bag with desiccant to minimize exposure to damp air.

Standard Curve

A typical standard curve with optical density readings at 450 nm on the Y-axis against IgM concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated in each experiment.