Rat IgM ELISA Kit

Name: Rat IgM ELISA Kit
SKU: DEIA681
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma

Species Reactivity

Rat

Detection Method

Sandwich-ELISA

Intended Use

This enzyme linked immunosorbent assay (ELISA) is for the detection of Rat IgM in serum or plasma. Due to idiotypic and allotypic variations, this product may not accurately quantitate hybridomas, monoclonals or myelomas.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Rat IgM Pre-Coated 96-well Strip Plate	1 each	2-8°C
2	Rat IgM Standard, 2000 ng/vial	2 each	2-8°C
3	Rat IgM Detection Antibody	12 ml	2-8°C
4	10× Dilution Buffer B	25 ml	2-8°C
5	HRP Solution A	12 ml	2-8°C
6	TMB Substrate	12 ml	2-8°C
7	Stop Solution	12 ml	2-8°C
8	20× Wash Buffer	50 ml	2-8°C
9	Sealing Tape	6 sheets

Storage

Stored at 2-8°C for 6 months from date of receipt.

General Description

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgG, and IgA. The rat IgG antibody has multiple functions including opsonization, complement function, and antibody-dependent cell mediated cytotoxicity. Rat IgG has been further categorized into subclasses: IgG1, IgG2a, IgG2b, and IgG2c. Functionally, Rat IgG1 and IgG2a have been shown to bind complement. This is in contrast to mice where only IgG2a binds complement (Overy 1966, Medgyesi 1974). In rats, as with other mammals, IgA functions primarily in mucosal immunity (Nash 1969). Rat IgM functions primarily as a naïve B-cell receptor and in complement functions. Concentrations of Rat IgM in serum are typically shown to be in the range of 0.6 to 1.0 mg/ml.

Standard Curve

This typical standard curve was generated using the Rat IgM ELISA Kit Protocol. This standard curve is for demonstration only. A standard curve must be generated for each assay.

*Suggested standard curve points are 2000 ng/ml, 667, 222, 74, 24.6, 8.2, 2.74, and 0 ng/ml. Assay Range: 2.74 – 2000 ng/ml

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Immunoassays for Process-related Impurities

QA & Reviews

Customer Reviews

References

Developmental expression of B cell molecules in equine lymphoid tissues

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

Authors: Prieto, J. M. B.; Tallmadge, R. L.; Felippe, M. J. B.

Abstract

Identification and classification of B cell subpopulations has been shown to be challenging and inconsistent among different species. Our study tested aspects of ontogeny, phenotype, tissue distribution, and function of equine CD5(hi) B cells, which represented a greater proportion of B cells early in development and in the peritoneal cavity. CD5(hi) and CD5(l0) B cells differentially expressed B cell markers (CD2, CD21, IgM) measured using flow cytometry, but similar mRNA expression of signature genes (DGKA, FGL2, PAX5, IGHM, IL10) measured using quantitative RT-PCR. Sequencing lambda light chain segments revealed that CD5(hi) B cells generated diverse immunoglobulin repertoires, and more frequently bound to fluorescence-labeled phosphorylcholine. This study shows developmental characteristics and tissue distribution of a newly described subpopulation of B cells in the horse. (C) 2016 Elsevier B.V. All rights reserved.

COMPARATIVE MAPPING OF IGHG1, IGHM, FES, AND FOS IN DOMESTIC CATTLE

IMMUNOGENETICS

Authors: TOBINJANZEN, TC; WOMACK, JE

Abstract

The immunoglobulin genes have not been genetically characterized as thoroughly in cattle as in other mammals, particularly humans and mice. Comparative gene mapping in mammals suggests that the bovine immunoglobulin heavy chain genes, IGHG4 and IGHM might be syntenic with the FOS oncogene. Interestingly, however, when these genes were assigned to bovine syntenic groups utilizing a panel of bovine: hamster hybrid somatic cells, IGH genes were shown to be syntenic with the FES oncogene rather than FOS. In this study IGH and FES were assigned to Bos taurus chromsome 21 while FOS was assigned to chromosome 10. In addition, bovine-specific immunoglobulin-like sequences were observed in the hybrid somatic cells, and one, IGHML1, was mapped to bovine syntenic group U16. The probes used for somatic-cell mapping were also used to screen a small number of cattle of several different breeds for restriction fragment length polymorphisms. IGHG4 and IGHM were shown to be highly polymorphic, while FOS and FES were not.