Rabbit IgM(Immunoglobulin M) ELISA Kit

Name: Rabbit IgM(Immunoglobulin M) ELISA Kit
SKU: DEIA8499
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Rabbit

Detection Method

Sandwich-ELISA

Intended Use

The kit is intended for in vitro quantitative determination of IgM concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	ELISA Microplate(Dismountable)	96T (8×12)	2-8°C/-20°C
2	Lyophilized Standard	2 vials	2-8°C/-20°C
3	HRP-labeled Antibody (Concentrated, 100×)	120ul	2-8°C (Avoid Direct Light)
4	TMB Substrate	10ml	2-8°C (Avoid Direct Light)
5	Sample Dilution Buffer	20ml	2-8°C
6	Antibody Dilution Buffer	10ml	2-8°C
7	Stop Solution	10ml	2-8°C
8	Wash Buffer(25×)	30ml	2-8°C
9	Plate Sealer	5 pieces

Storage

2-8°C for 6 months

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Detection Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Immunoassays for Process-related Impurities

QA & Reviews

Customer Reviews

References

Structure of the genomic sequence comprising the immunoglobulin heavy constant (IGHC) genes from Sus scrofa

MOLECULAR IMMUNOLOGY

Authors: Eguchi-Ogawa, Tomoko; Toki, Daisuke; Wertz, Nancy; Butler, John E.; Uenishi, Hirohide

Abstract

The number of immunoglobulin heavy chain (IGH) constant genes (IGHC) varies among mammals. To annotate the porcine IGHC genes, we sequenced the entire IGHC-containing genomic region from a single porcine haplotype. The resulting contiguous sequence included in 5' the IGH diversity (D) gene cluster and in 3' TMEM121, which flank the IGHC cluster in the human genome, suggesting that we had obtained the entire genomic region containing porcine IGHC. This region was about 190-kb long, in good agreement with those of other mammals. The porcine IGHC cluster contained 10 genes, IGHM, IGHD, six IGHG genes, IGHE and IGHA. The porcine IGHG genes formed a cluster between IGHD and IGHE, with IGHG3 considered as the most ancient IGHG gene, located at the beginning of the IGHG cluster. Furthermore, the porcine sequence contained two IGHG5 and two IGHG6 genes, but no IGHG genes for IgG2 and IgG4, suggesting flexibility within the IGHG cluster. We also recorded structural differences in the switch regions of the IGHC genes that may be important in their transcription. This haplotype can serve as a reference for future studies on other haplotypes and for functional analysis of porcine immunoglobulin (IG) isotypes. (C) 2012 Elsevier Ltd. All rights reserved.

COMPARATIVE MAPPING OF IGHG1, IGHM, FES, AND FOS IN DOMESTIC CATTLE

IMMUNOGENETICS

Authors: TOBINJANZEN, TC; WOMACK, JE

Abstract

The immunoglobulin genes have not been genetically characterized as thoroughly in cattle as in other mammals, particularly humans and mice. Comparative gene mapping in mammals suggests that the bovine immunoglobulin heavy chain genes, IGHG4 and IGHM might be syntenic with the FOS oncogene. Interestingly, however, when these genes were assigned to bovine syntenic groups utilizing a panel of bovine: hamster hybrid somatic cells, IGH genes were shown to be syntenic with the FES oncogene rather than FOS. In this study IGH and FES were assigned to Bos taurus chromsome 21 while FOS was assigned to chromosome 10. In addition, bovine-specific immunoglobulin-like sequences were observed in the hybrid somatic cells, and one, IGHML1, was mapped to bovine syntenic group U16. The probes used for somatic-cell mapping were also used to screen a small number of cattle of several different breeds for restriction fragment length polymorphisms. IGHG4 and IGHM were shown to be highly polymorphic, while FOS and FES were not.