Sample
Serum, plasma and egg-yolk extracts
Species Reactivity
Turkey
Detection Method
Sandwich-ELISA
Intended Use
This kit can be used for quantitative measurement of IgM in turkey serum, plasma and egg-yolk extracts.
Contents of Kit
| No. | Components | Size | Storage Conditions |
| 1 | Anti Turkey IgM Coated 96-well Plate | 12×8 wells | 2-8°C |
| 2 | HRP Conjugate Reagent | 11 ml | 2-8°C |
| 3 | Reference Standard (lyophilized) | 1 | 2-8°C |
| 4 | 20× Wash Solution | 50 ml | 2-8°C |
| 5 | 10× Immunoglobulin Diluent | 25 ml | 2-8°C |
| 6 | TMB Reagent (One-Step) | 11 ml | 2-8°C |
| 7 | Stop Solution (1N HCl) | 11 ml | 2-8°C |
Storage
The test kit will remain stable for six months from the date of purchase provided that the components are stored at 2-8°C. The microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air.
Standard Curve
A typical standard curve with optical density readings at 450nm on the Y-axis against IgM concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain his or her data and standard curve in each experiment.

Citations
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Bengten, E; Quiniou, S; et al. Structure of the catfish IGH locus: analysis of the region including the single functional IGHM gene. IMMUNOGENETICS 58:831-844(2006).
Magor, KE; et al. Immunoglobulin genetics and antibody responses to influenza in ducks. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35:1008-1017(2011).