Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing
PLOS ONE
Authors: Vollmers, Christopher; Penland, Lolita; Kanbar, Jad N.; Quake, Stephen R.
Abstract
Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single molecule sequencing of antibody heavy chain transcripts and were able to identify novel exons for IGHA1 and IGHA2 as well as novel isoforms for IGHM antibody heavy chain.
Developmental expression of B cell molecules in equine lymphoid tissues
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Authors: Prieto, J. M. B.; Tallmadge, R. L.; Felippe, M. J. B.
Abstract
Identification and classification of B cell subpopulations has been shown to be challenging and inconsistent among different species. Our study tested aspects of ontogeny, phenotype, tissue distribution, and function of equine CD5(hi) B cells, which represented a greater proportion of B cells early in development and in the peritoneal cavity. CD5(hi) and CD5(l0) B cells differentially expressed B cell markers (CD2, CD21, IgM) measured using flow cytometry, but similar mRNA expression of signature genes (DGKA, FGL2, PAX5, IGHM, IL10) measured using quantitative RT-PCR. Sequencing lambda light chain segments revealed that CD5(hi) B cells generated diverse immunoglobulin repertoires, and more frequently bound to fluorescence-labeled phosphorylcholine. This study shows developmental characteristics and tissue distribution of a newly described subpopulation of B cells in the horse. (C) 2016 Elsevier B.V. All rights reserved.
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