Rhesus Macaque ICAM-1 Protein [Fc]

Product Overview

Recombinant Intercellular adhesion molecule 1 is produced by our Mammalian expression system and the target gene encoding Gln28-Glu480 is expressed with a Fc tag at the C-terminus.

Nature

Recombinant

Tag/Conjugate

Fc

Endotoxin

Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Alternative Names

Intercellular adhesion molecule 1; ICAM1; ICAM-1; CD54

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Format

Lyophilized

Buffer

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Preservative

None

Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Introduction

Intercellular adhesion molecule 1 (ICAM1) is known as CD54. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

Keywords

Intercellular adhesion molecule 1; ICAM1; ICAM-1; CD54